KATNB1 sirna

KATNB1 siRNA (Human)

Cat. Num. AAA8219210

• Gene Names: KATNB1; KAT; LIS6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KATNB1; KATNB1 siRNA (Human); Katanin p80 WD40 repeat-containing subunit B1; Katanin p80 subunit B1; p80 katanin; KATNB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KATNB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 655

• Applicable Applications for KATNB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KATNB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KATNB1 sirna

siRNA to inhibit KATNB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 83779014
• NCBI GeneID: 10300
• NCBI Accession #: NP_005877.2
• NCBI GenBank Nucleotide #: NM_005886.2
• UniProt Accession #: Q9BVA0; O60620; A6NCG6
• Molecular Weight: 72,334 Da
• NCBI Official Full Name: katanin p80 WD40 repeat-containing subunit B1
• NCBI Official Synonym Full Names: katanin p80 (WD repeat containing) subunit B 1
• NCBI Official Symbol: KATNB1
• NCBI Official Synonym Symbols: KAT; LIS6
• NCBI Protein Information: katanin p80 WD40 repeat-containing subunit B1
• UniProt Protein Name: Katanin p80 WD40 repeat-containing subunit B1
• Protein Family: KATNB1-like protein
• UniProt Gene Name: KATNB1
• UniProt Synonym Gene Names: Katanin p80 subunit B1
• UniProt Entry Name: KTNB1_HUMAN

NCBI Description

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases. [provided by RefSeq, Jul 2008]

Uniprot Description

KATNB1: Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth. Belongs to the WD repeat KATNB1 family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: microtubule cytoskeleton; spindle pole; centrosome; microtubule; growth cone; membrane; cell soma; katanin complex; axon; cytoplasm; plasma membrane; midbody; nucleus

Molecular Function: dynein binding; protein heterodimerization activity; microtubule binding; microtubule-severing ATPase activity

Biological Process: positive regulation of microtubule depolymerization; cell division; metabolic process; negative regulation of microtubule depolymerization; microtubule severing; mitotic chromosome movement towards spindle pole; protein targeting

Disease: Lissencephaly 6, With Microcephaly