KAT6B sirna

KAT6B siRNA (Human)

Cat. Num. AAA8241392

• Gene Names: KAT6B; qkf; MORF; MOZ2; GTPTS; MYST4; ZC2HC6B; querkopf
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KAT6B; KAT6B siRNA (Human); KIAA0383; MORF; MOZ2; MYST4; Histone acetyltransferase KAT6B; Histone acetyltransferase MOZ2; MOZ. YBF2/SAS3. SAS2 and TIP60 protein 4; MYST-4; Monocytic leukemia zinc finger protein-related factor; KAT6B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KAT6B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1890

• Applicable Applications for KAT6B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KAT6B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KAT6B sirna

siRNA to inhibit KAT6B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 374349205
• NCBI GeneID: 23522
• NCBI Accession #: NP_001243397.1
• NCBI GenBank Nucleotide #: NM_001256468.1
• UniProt Accession #: Q8WYB5; O15087; Q86Y05; Q8WU81; Q9UKW2; Q9UKW3; Q9UKX0
• Molecular Weight: 199,858 Da
• NCBI Official Full Name: histone acetyltransferase KAT6B isoform 2
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 6B
• NCBI Official Symbol: KAT6B
• NCBI Official Synonym Symbols: qkf; MORF; MOZ2; GTPTS; MYST4; ZC2HC6B; querkopf
• NCBI Protein Information: histone acetyltransferase KAT6B
• UniProt Protein Name: Histone acetyltransferase KAT6B
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: KAT6B
• UniProt Synonym Gene Names: KIAA0383; MORF; MOZ2; MYST4; MYST-4
• UniProt Entry Name: KAT6B_HUMAN

NCBI Description

The protein encoded by this gene is a histone acetyltransferase and component of the MOZ/MORF protein complex. In addition to its acetyltransferase activity, the encoded protein has transcriptional activation activity in its N-terminal end and transcriptional repression activity in its C-terminal end. This protein is necessary for RUNX2-dependent transcriptional activation and could be involved in brain development. Mutations have been found in patients with genitopatellar syndrome. A translocation of this gene and the CREBBP gene results in acute myeloid leukemias. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

MYST4: Histone acetyltransferase which may be involved in both positive and negative regulation of transcription. Required for RUNX2-dependent transcriptional activation. May be involved in cerebral cortex development. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. A chromosomal aberration involving KAT6B may be a cause acute myeloid leukemias. Translocation t(10;16)(q22;p13) with CREBBP. Defects in KAT6B are a cause of Ohdo syndrome, SBBYS variant (SBBYSS). SBBYSS is a syndrome characterized by distinctive facial appearance with severe blepharophimosis, an immobile mask-like face, a bulbous nasal tip, and a small mouth with a thin upper lip. The condition presents in infancy with severe hypotonia and feeding problems. Associated skeletal problems include joint laxity, abnormally long thumbs and great toes, and dislocated or hypoplastic patellae. Structural cardiac defects are present in around 50% of cases, and dental anomalies, including small and pointed teeth, are common. Many affected individuals have abnormalities of thyroid structure or function. SBBYSS is usually associated with severe mental retardation, delayed motor milestones, and significantly impaired speech. Defects in KAT6B are a cause of genitopatellar syndrome (GTPTS). GTPTS is a rare disorder consisting of microcephaly, severe psychomotor retardation, and characteristic coarse facial features, including broad nose and small or retracted chin, associated with congenital flexion contractures of the lower extremities, abnormal or missing patellae, and urogenital anomalies. Belongs to the MYST (SAS/MOZ) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.48; Acetyltransferase

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: nucleoplasm; nucleosome; nucleus

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; zinc ion binding; acetyltransferase activity; transcription factor binding

Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone acetylation; negative regulation of transcription, DNA-dependent

Disease: Genitopatellar Syndrome; Ohdo Syndrome, Sbbys Variant