INPP5K sirna

INPP5K siRNA (Human)

Cat. Num. AAA8222776

• Gene Names: INPP5K; PPS; SKIP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: INPP5K; INPP5K siRNA (Human); PPS; SKIP; Inositol polyphosphate 5-phosphatase K; Skeletal muscle and kidney-enriched inositol phosphatase; INPP5K sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: INPP5K siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 372

• Applicable Applications for INPP5K sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human INPP5K gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for INPP5K sirna

siRNA to inhibit INPP5K expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 208610044
• NCBI GeneID: 51763
• NCBI Accession #: NP_001129114.1
• NCBI GenBank Nucleotide #: NM_001135642.1
• UniProt Accession #: Q9BT40; Q15733; Q9NPJ5; Q9P2R5; B2R6I2; B2R750; D3DTH8
• Molecular Weight: 42,784 Da
• NCBI Official Full Name: inositol polyphosphate 5-phosphatase K isoform 2
• NCBI Official Synonym Full Names: inositol polyphosphate-5-phosphatase K
• NCBI Official Symbol: INPP5K
• NCBI Official Synonym Symbols: PPS; SKIP
• NCBI Protein Information: inositol polyphosphate 5-phosphatase K
• UniProt Protein Name: Inositol polyphosphate 5-phosphatase K
• Protein Family: Inositol polyphosphate 5-phosphatase
• UniProt Gene Name: INPP5K
• UniProt Synonym Gene Names: PPS; SKIP
• UniProt Entry Name: INP5K_HUMAN

NCBI Description

This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

Uniprot Description

INPP5K: Inositol 5-phosphatase which acts on inositol 1,4,5- trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5- trisphosphate. May negatively regulate assembly of the actin cytoskeleton. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - inositol phosphate; Phosphatase (non-protein); EC 3.1.3.56

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: ruffle; neuron projection; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; plasma membrane; trans-Golgi network; nucleus; cytosol

Molecular Function: protein binding; inositol trisphosphate phosphatase activity; lipid phosphatase activity; inositol-polyphosphate 5-phosphatase activity; inositol bisphosphate phosphatase activity; vasopressin receptor activity

Biological Process: negative regulation of MAP kinase activity; positive regulation of transcription, DNA-dependent; regulation of glycogen biosynthetic process; negative regulation of peptidyl-serine phosphorylation; negative regulation of insulin receptor signaling pathway; glucose homeostasis; negative regulation of protein amino acid phosphorylation; negative regulation of dephosphorylation; phospholipid metabolic process; negative regulation of glycogen biosynthetic process; negative regulation of retroviral genome replication; negative regulation of calcium ion transport; negative regulation of protein kinase B signaling cascade; negative regulation of stress fiber formation; in utero embryonic development; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; cellular response to insulin stimulus; dephosphorylation; inositol phosphate dephosphorylation; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; actin cytoskeleton organization and biogenesis