IER3 sirna

IER3 siRNA (Human)

Cat. Num. AAA8233545

• Gene Names: IER3; DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IER3; IER3 siRNA (Human); DIF2; IEX1; PRG1; Radiation-inducible immediate-early gene IEX-1; Differentiation-dependent gene 2 protein; Protein DIF-2; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein; Protein PRG1; IER3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IER3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 156

• Applicable Applications for IER3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IER3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IER3 sirna

siRNA to inhibit IER3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 119964723
• NCBI GeneID: 8870
• NCBI Accession #: NP_003888.2
• NCBI GenBank Nucleotide #: NM_003897.3
• UniProt Accession #: P46695; Q5SU30; Q92691; Q93044
• Molecular Weight: 16,903 Da
• NCBI Official Full Name: radiation-inducible immediate-early gene IEX-1
• NCBI Official Synonym Full Names: immediate early response 3
• NCBI Official Symbol: IER3
• NCBI Official Synonym Symbols: DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
• NCBI Protein Information: radiation-inducible immediate-early gene IEX-1
• UniProt Protein Name: Radiation-inducible immediate-early gene IEX-1
• Protein Family: Radiation-inducible immediate-early gene
• UniProt Gene Name: IER3
• UniProt Synonym Gene Names: DIF2; IEX1; PRG1; Protein DIF-2; Protein PRG1
• UniProt Entry Name: IEX1_HUMAN

NCBI Description

This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. [provided by RefSeq, Jul 2008]

Uniprot Description

IEX-1: May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. Belongs to the IER3 family.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to membrane; nucleus

Molecular Function: protein binding

Biological Process: regulation of DNA repair; anatomical structure morphogenesis; response to protozoan; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; negative regulation of systemic arterial blood pressure; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of inflammatory response; regulation of nucleocytoplasmic transport; positive regulation of protein catabolic process; negative regulation of glycolysis; negative regulation of apoptosis