HOOK1 sirna

HOOK1 siRNA (Human)

Cat. Num. AAA8229741

• Gene Names: HOOK1; HK1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HOOK1; HOOK1 siRNA (Human); Protein Hook homolog 1; h-hook1; hHK1; HOOK1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HOOK1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 728

• Applicable Applications for HOOK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HOOK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships on blue ice. Store at -20 degree C for one year.

Related Product Information for HOOK1 sirna

siRNA to inhibit HOOK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7705415
• NCBI GeneID: 51361
• NCBI Accession #: NP_056972.1
• NCBI GenBank Nucleotide #: NM_015888.4
• UniProt Accession #: Q9UJC3; O60561; Q5TG44; A8K8E9; A8MU44; B4DX15
• NCBI Official Full Name: protein Hook homolog 1
• NCBI Official Synonym Full Names: hook microtubule-tethering protein 1
• NCBI Official Symbol: HOOK1
• NCBI Official Synonym Symbols: HK1
• NCBI Protein Information: protein Hook homolog 1
• UniProt Protein Name: Protein Hook homolog 1
• Protein Family: Protein Hook
• UniProt Gene Name: HOOK1
• UniProt Synonym Gene Names: h-hook1; hHK1
• UniProt Entry Name: HOOK1_HUMAN

NCBI Description

This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HOOK1: Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Belongs to the hook family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1p32.1

Cellular Component: microtubule

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: protein transport; endosome organization and biogenesis; lysosome organization and biogenesis; early endosome to late endosome transport; multicellular organismal development; endosome to lysosome transport; spermatid development