HNRNPDL sirna

HNRNPDL siRNA (Human)

Cat. Num. AAA8237587

• Gene Names: HNRNPDL; HNRNP; JKTBP; HNRPDL; JKTBP2; LGMD1G; laAUF1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HNRNPDL; HNRNPDL siRNA (Human); HNRPDL; JKTBP; Heterogeneous nuclear ribonucleoprotein D-like; hnRNP D-like; hnRNP DL; AU-rich element RNA-binding factor; JKT41-binding protein; Protein laAUF1; HNRNPDL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HNRNPDL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 244

• Applicable Applications for HNRNPDL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HNRNPDL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HNRNPDL sirna

siRNA to inhibit HNRNPDL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 332801090
• NCBI GeneID: 9987
• NCBI Accession #: NP_001193929.1
• NCBI GenBank Nucleotide #: NM_001207000.1
• UniProt Accession #: O14979; Q6SPF2; Q7KZ74; Q7KZ75; Q96IM0; Q96S43
• Molecular Weight: 27,191 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein D-like isoform b
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein D-like
• NCBI Official Symbol: HNRNPDL
• NCBI Official Synonym Symbols: HNRNP; JKTBP; HNRPDL; JKTBP2; LGMD1G; laAUF1
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein D-like
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein D-like
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPDL
• UniProt Synonym Gene Names: HNRPDL; JKTBP; hnRNP D-like; hnRNP DL
• UniProt Entry Name: HNRDL_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind to RNAs. Three alternatively spliced transcript variants have been described for this gene. One of the variants is probably not translated because the transcript is a candidate for nonsense-mediated mRNA decay. The protein isoforms encoded by this gene are similar to its family member HNRPD. [provided by RefSeq, May 2011]

Uniprot Description

hnRNP D-like: Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes. Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter. Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA processing; DNA-binding

Chromosomal Location of Human Ortholog: 4q21.22

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleus

Molecular Function: DNA binding; double-stranded DNA binding; nucleotide binding; poly(rG) binding; single-stranded DNA binding; poly(A) binding

Biological Process: RNA processing; transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of gene expression

Disease: Limb-girdle Muscular Dystrophy, Type 1g