HIVEP3 sirna

HIVEP3 siRNA (Human)

Cat. Num. AAA827154

• Gene Names: HIVEP3; KRC; KBP1; SHN3; ZAS3; KBP-1; ZNF40C; Schnurri-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HIVEP3; HIVEP3 siRNA (Human); KBP1; KIAA1555; KRC; ZAS3; Transcription factor HIVEP3; Human immunodeficiency virus type I enhancer-binding protein 3; Kappa-B and V(D)J recombination signal sequences-binding protein; Kappa-binding protein 1; KBP-1; Zinc finger protein ZAS3; HIVEP3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HIVEP3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2405

• Applicable Applications for HIVEP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HIVEP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HIVEP3 sirna

siRNA to inhibit HIVEP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 189181750
• NCBI GeneID: 59269
• NCBI Accession #: NP_001121186.1
• NCBI GenBank Nucleotide #: NM_001127714.2
• UniProt Accession #: Q5T1R4; Q5T1R5; Q9BZS0; Q9HCL7; A7YY91
• Molecular Weight: 259,336 Da
• NCBI Official Full Name: transcription factor HIVEP3 isoform b
• NCBI Official Synonym Full Names: human immunodeficiency virus type I enhancer binding protein 3
• NCBI Official Symbol: HIVEP3
• NCBI Official Synonym Symbols: KRC; KBP1; SHN3; ZAS3; KBP-1; ZNF40C; Schnurri-3
• NCBI Protein Information: transcription factor HIVEP3
• UniProt Protein Name: Transcription factor HIVEP3
• Protein Family: Transcription factor
• UniProt Gene Name: HIVEP3
• UniProt Synonym Gene Names: KBP1; KIAA1555; KRC; ZAS3; KBP-1
• UniProt Entry Name: ZEP3_HUMAN

NCBI Description

This gene encodes a member of the human immunodeficiency virus type 1 enhancer-binding protein family. Members of this protein family contain multiple zinc finger and acid-rich (ZAS) domains and serine-threonine rich regions. This protein acts as a transcription factor and is able to regulate nuclear factor kappaB-mediated transcription by binding the kappaB motif in target genes. This protein also binds the recombination signal sequence that flanks the V, D, and J regions of immunoglobulin and T-cell receptors. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

HIVEP3: Plays a role of transcription factor; binds to recognition signal sequences (Rss heptamer) for somatic recombination of immunoglobulin and T-cell receptor gene segments; Binds also to the kappa-B motif of gene such as S100A4, involved in cell progression and differentiation. Kappa-B motif is a gene regulatory element found in promoters and enhancers of genes involved in immunity, inflammation, and growth and that responds to viral antigens, mitogens, and cytokines. Involvement of HIVEP3 in cell growth is strengthened by the fact that its down- regulation promotes cell cycle progression with ultimate formation of multinucleated giant cells. Strongly inhibits TNF-alpha-induced NF-kappa-B activation; Interferes with nuclear factor NF-kappa-B by several mechanisms: as transcription factor, by competing for Kappa-B motif and by repressing transcription in the nucleus; through a non transcriptional process, by inhibiting nuclear translocation of RELA by association with TRAF2, an adapter molecule in the tumor necrosis factor signaling, which blocks the formation of IKK complex. Interaction with TRAF proteins inhibits both NF-Kappa-B-mediated and c-Jun N-terminal kinase/JNK-mediated responses that include apoptosis and proinflammatory cytokine gene expression. Positively regulates the expression of IL2 in T-cell. Essential regulator of adult bone formation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent