HIST1H4G sirna

HIST1H4G siRNA (Human)

Cat. Num. AAA8211218

• Gene Names: HIST1H4G; H4/l; H4FL
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HIST1H4G; HIST1H4G siRNA (Human); H4/L; H4FL; Histone H4-like protein type G; HIST1H4G sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HIST1H4G siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 98

• Applicable Applications for HIST1H4G sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HIST1H4G gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HIST1H4G sirna

siRNA to inhibit HIST1H4G expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504319
• NCBI GeneID: 8369
• NCBI Accession #: NP_003538.1
• NCBI GenBank Nucleotide #: NM_003547.2
• UniProt Accession #: Q99525
• Molecular Weight: 11,009 Da
• NCBI Official Full Name: histone H4-like protein type G
• NCBI Official Synonym Full Names: histone cluster 1, H4g
• NCBI Official Symbol: HIST1H4G
• NCBI Official Synonym Symbols: H4/l; H4FL
• NCBI Protein Information: histone H4-like protein type G
• UniProt Protein Name: Histone H4-like protein type G
• Protein Family: Histone H4-like protein
• UniProt Gene Name: HIST1H4G
• UniProt Synonym Gene Names: H4/L; H4FL
• UniProt Entry Name: H4G_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Uniprot Description

H4G: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Belongs to the histone H4 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleosome; nucleus

Molecular Function: DNA binding; protein heterodimerization activity