HIC1 sirna

HIC1 siRNA (Human)

Cat. Num. AAA8212644

• Gene Names: HIC1; hic-1; ZBTB29; ZNF901
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HIC1; HIC1 siRNA (Human); ZBTB29; Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HIC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 733

• Applicable Applications for HIC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HIC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HIC1 sirna

siRNA to inhibit HIC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148237270
• NCBI GeneID: 3090
• NCBI Accession #: NP_001091672.1
• NCBI GenBank Nucleotide #: NM_001098202.1
• UniProt Accession #: Q14526; D3DTI4
• Molecular Weight: 74,514 Da
• NCBI Official Full Name: hypermethylated in cancer 1 protein isoform 2
• NCBI Official Synonym Full Names: hypermethylated in cancer 1
• NCBI Official Symbol: HIC1
• NCBI Official Synonym Symbols: hic-1; ZBTB29; ZNF901
• NCBI Protein Information: hypermethylated in cancer 1 protein
• UniProt Protein Name: Hypermethylated in cancer 1 protein
• Protein Family: Hypermethylated in cancer 1 protein
• UniProt Gene Name: HIC1
• UniProt Synonym Gene Names: ZBTB29; Hic-1
• UniProt Entry Name: HIC1_HUMAN

NCBI Description

This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Uniprot Description

HIC1: Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF- responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from CXCR7, FGFBP1 and EFNA1. Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: nucleoplasm; cytoplasm; chromatin

Molecular Function: protein binding; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; transcription factor activity

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; transcription, DNA-dependent; regulation of transcription, DNA-dependent; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator