GUCY1B3 sirna

GUCY1B3 siRNA (Human)

Cat. Num. AAA8205234

• Gene Names: GUCY1B3; GUCB3; GC-SB3; GUC1B3; GUCSB3; GUCY1B1; GC-S-beta-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GUCY1B3; GUCY1B3 siRNA (Human); GUC1B3; GUCSB3; GUCY1B1; Guanylate cyclase soluble subunit beta-1; GCS-beta-1; Guanylate cyclase soluble subunit beta-3; GCS-beta-3; Soluble guanylate cyclase small subunit; GUCY1B3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GUCY1B3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 619

• Applicable Applications for GUCY1B3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GUCY1B3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GUCY1B3 sirna

siRNA to inhibit GUCY1B3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504215
• NCBI GeneID: 2983
• NCBI Accession #: NP_000848.1
• NCBI GenBank Nucleotide #: NM_000857.3
• UniProt Accession #: Q02153; Q86WY5; B7Z426
• Molecular Weight: 68,165 Da
• NCBI Official Full Name: guanylate cyclase soluble subunit beta-1 isoform 2
• NCBI Official Synonym Full Names: guanylate cyclase 1, soluble, beta 3
• NCBI Official Symbol: GUCY1B3
• NCBI Official Synonym Symbols: GUCB3; GC-SB3; GUC1B3; GUCSB3; GUCY1B1; GC-S-beta-1
• NCBI Protein Information: guanylate cyclase soluble subunit beta-1
• UniProt Protein Name: Guanylate cyclase soluble subunit beta-1
• Protein Family: Guanylate cyclase soluble
• UniProt Gene Name: GUCY1B3
• UniProt Synonym Gene Names: GUC1B3; GUCSB3; GUCY1B1; GCS-beta-1; GCS-beta-3
• UniProt Entry Name: GCYB1_HUMAN

NCBI Description

This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

GUCY1B3: Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Heterodimer of an alpha and a beta chain. 2 isoforms of the human protein are produced by alternative splicing

Protein type: Nucleotide Metabolism - purine; Receptor, misc.; Lyase; Guanylyl cyclase; EC 4.6.1.2

Chromosomal Location of Human Ortholog: 4q31.3-q33

Cellular Component: guanylate cyclase complex, soluble; intracellular membrane-bound organelle; cytoplasm

Molecular Function: guanylate cyclase activity; GTP binding; protein heterodimerization activity; metal ion binding; heme binding; receptor activity; Hsp90 protein binding

Biological Process: cGMP biosynthetic process; blood circulation; blood coagulation; nitric oxide mediated signal transduction