GPAM sirna

GPAM siRNA (Human)

Cat. Num. AAA8213033

• Gene Names: GPAM; GPAT; GPAT1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GPAM; GPAM siRNA (Human); GPAT1; KIAA1560; Glycerol-3-phosphate acyltransferase 1 mitochondrial; GPAT-1; GPAM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GPAM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 828

• Applicable Applications for GPAM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GPAM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GPAM sirna

siRNA to inhibit GPAM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 350276140
• NCBI GeneID: 57678
• NCBI Accession #: NP_001231878.1
• NCBI GenBank Nucleotide #: NM_001244949.1
• UniProt Accession #: Q9HCL2; Q5VW51; Q86TA3
• Molecular Weight: 93,795 Da
• NCBI Official Full Name: glycerol-3-phosphate acyltransferase 1, mitochondrial
• NCBI Official Synonym Full Names: glycerol-3-phosphate acyltransferase, mitochondrial
• NCBI Official Symbol: GPAM
• NCBI Official Synonym Symbols: GPAT; GPAT1
• NCBI Protein Information: glycerol-3-phosphate acyltransferase 1, mitochondrial
• UniProt Protein Name: Glycerol-3-phosphate acyltransferase 1, mitochondrial
• UniProt Gene Name: GPAM
• UniProt Synonym Gene Names: GPAT1; KIAA1560; GPAT-1
• UniProt Entry Name: GPAT1_HUMAN

NCBI Description

This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

GPAM: glycerol-3-phosphate acyltransferase is an integral multi-pass enzyme of the mitochondrial outer membrane. It performs the first step in de novo phospholipid biosynthesis. Member of the phospholipid and glycerol acyltransferase family

Protein type: Transferase; Mitochondrial; Lipid Metabolism - glycerolipid; Membrane protein, integral; Lipid Metabolism - glycerophospholipid; EC 2.3.1.15; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: mitochondrial outer membrane; mitochondrial inner membrane; integral to membrane; plasma membrane

Molecular Function: glycerol-3-phosphate O-acyltransferase activity

Biological Process: regulation of cytokine secretion; acyl-CoA metabolic process; glycerophospholipid biosynthetic process; triacylglycerol biosynthetic process; positive regulation of multicellular organism growth; cellular lipid metabolic process; fatty acid metabolic process; positive regulation of activated T cell proliferation; phospholipid metabolic process; fatty acid homeostasis; response to glucose stimulus; phosphatidic acid biosynthetic process; CDP-diacylglycerol biosynthetic process; defense response to virus; phospholipid homeostasis