GLO1 sirna

GLO1 siRNA (Human)

Cat. Num. AAA8236752

• Gene Names: GLO1; GLYI; GLOD1; HEL-S-74
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GLO1; GLO1 siRNA (Human); Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; GLO1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GLO1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 169

• Applicable Applications for GLO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GLO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GLO1 sirna

siRNA to inhibit GLO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118402586
• NCBI GeneID: 2739
• NCBI Accession #: NP_006699.2
• NCBI GenBank Nucleotide #: NM_006708.2
• UniProt Accession #: Q04760; P78375; Q59EL0; Q5TZW3; Q96FC0; Q96J41; B2R6P7; B4DDV0
• Molecular Weight: 19,043 Da
• NCBI Official Full Name: lactoylglutathione lyase
• NCBI Official Synonym Full Names: glyoxalase I
• NCBI Official Symbol: GLO1
• NCBI Official Synonym Symbols: GLYI; GLOD1; HEL-S-74
• NCBI Protein Information: lactoylglutathione lyase
• UniProt Protein Name: Lactoylglutathione lyase
• UniProt Gene Name: GLO1
• UniProt Synonym Gene Names: Glx I
• UniProt Entry Name: LGUL_HUMAN

NCBI Description

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

Uniprot Description

GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.4.1.5; Lyase; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 6p21.3-p21.1

Cellular Component: cytoplasm

Molecular Function: zinc ion binding; lactoylglutathione lyase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; negative regulation of apoptosis; methylglyoxal metabolic process

Disease: Autism