GHR sirna

GHR siRNA (Human)

Cat. Num. AAA8229259

• Gene Names: GHR; GHBP; GHIP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GHR; GHR siRNA (Human); Growth hormone receptor; GH receptor; Somatotropin receptor; GHR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GHR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 638

• Applicable Applications for GHR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GHR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GHR sirna

siRNA to inhibit GHR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503993
• NCBI GeneID: 2690
• NCBI Accession #: NP_000154.1
• NCBI GenBank Nucleotide #: NM_000163.4
• UniProt Accession #: P10912; Q9HCX2
• Molecular Weight: 69,237 Da
• NCBI Official Full Name: growth hormone receptor isoform 1
• NCBI Official Synonym Full Names: growth hormone receptor
• NCBI Official Symbol: GHR
• NCBI Official Synonym Symbols: GHBP; GHIP
• NCBI Protein Information: growth hormone receptor
• UniProt Protein Name: Growth hormone receptor
• Protein Family: Ghrelin
• UniProt Gene Name: GHR
• UniProt Synonym Gene Names: GH receptor; GH-binding protein; GHBP
• UniProt Entry Name: GHR_HUMAN

NCBI Description

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

GH receptor: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Defects in GHR are a cause of Laron syndrome (LARS). A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the type I cytokine receptor family. Type 1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; integral to membrane; plasma membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; peptide hormone binding; growth factor binding; protein kinase binding

Biological Process: succinate metabolic process; oxaloacetate metabolic process; activation of MAPK activity; positive regulation of multicellular organism growth; fatty acid metabolic process; tyrosine phosphorylation of JAK2 protein; response to estradiol stimulus; 2-oxoglutarate metabolic process; positive regulation of tyrosine phosphorylation of Stat3 protein; allantoin metabolic process; receptor internalization; creatinine metabolic process; isoleucine metabolic process; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; citrate metabolic process; valine metabolic process; endocytosis; JAK-STAT cascade; creatine metabolic process; multicellular organismal metabolic process; cellular response to hormone stimulus; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat5 protein; response to cycloheximide; taurine metabolic process

Disease: Hypercholesterolemia, Familial; Laron Syndrome