GEMIN5 sirna

GEMIN5 siRNA (Human)

Cat. Num. AAA8221828

• Gene Names: GEMIN5; GEMIN-5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GEMIN5; GEMIN5 siRNA (Human); Gem-associated protein 5; Gemin5; GEMIN5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GEMIN5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1507

• Applicable Applications for GEMIN5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GEMIN5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GEMIN5 sirna

siRNA to inhibit GEMIN5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 356461016
• NCBI GeneID: 25929
• NCBI Accession #: NP_001239085.1
• NCBI GenBank Nucleotide #: NM_001252156.1
• UniProt Accession #: Q8TEQ6; Q14CV0; Q8WWV4; Q969W4; Q9H9K3; Q9UFI5
• Molecular Weight: 168,589 Da
• NCBI Official Full Name: gem-associated protein 5 isoform 2
• NCBI Official Synonym Full Names: gem (nuclear organelle) associated protein 5
• NCBI Official Symbol: GEMIN5
• NCBI Official Synonym Symbols: GEMIN-5
• NCBI Protein Information: gem-associated protein 5
• UniProt Protein Name: Gem-associated protein 5
• Protein Family: Gem-associated protein
• UniProt Gene Name: GEMIN5
• UniProt Synonym Gene Names: Gemin5
• UniProt Entry Name: GEMI5_HUMAN

NCBI Description

This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

GEMIN5: the snRNA-binding protein of the SMN complex. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.

Protein type: Nuclear receptor co-regulator; RNA splicing; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 5q33.2

Cellular Component: nucleoplasm; nuclear body; membrane; cytoplasm; SMN complex; cytosol; nucleus

Molecular Function: protein binding; snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; protein complex assembly; gene expression