GALNT4 sirna

GALNT4 siRNA (Human)

Cat. Num. AAA8236804

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GALNT4; GALNT4 siRNA (Human); Polypeptide N-acetylgalactosaminyltransferase 4; Polypeptide GalNAc transferase 4; GalNAc-T4; pp-GaNTase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4; GALNT4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: GALNT4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 406

• Applicable Applications for GALNT4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GALNT4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GALNT4 sirna

siRNA to inhibit GALNT4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 315139010
• NCBI GeneID: 100528030
• NCBI Accession #: NP_001186711.1
• NCBI GenBank Nucleotide #: NM_001199782.1
• UniProt Accession #: Q8N4A0; O00208; B2R775; B4DMX6
• Molecular Weight: 45,702 Da
• NCBI Official Full Name: POC1B-GALNT4 protein isoform 2
• NCBI Official Synonym Full Names: POC1B-GALNT4 readthrough
• NCBI Official Symbol: POC1B-GALNT4
• NCBI Protein Information: POC1B-GALNT4 protein
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 4
• Protein Family: Polypeptide N-acetylgalactosaminyltransferase
• UniProt Gene Name: GALNT4
• UniProt Synonym Gene Names: GalNAc-T4; pp-GaNTase 4
• UniProt Entry Name: GALT4_HUMAN

NCBI Description

This locus represents naturally occurring transcripts that splice the 5' exons of the POC1B (POC1 centriolar protein homolog B) gene on chromosome 12 to the GALNT4 (UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4) gene, which is located within a POC1B intron. Alternative splicing results in two transcript variants, one of which encodes a fusion isoform that shares sequence identity with the products of each individual gene. [provided by RefSeq, Dec 2010]

Uniprot Description

GALNT4: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane.

Protein type: Transferase; Membrane protein, integral; EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: Golgi membrane; Golgi apparatus; perinuclear region of cytoplasm; integral to membrane

Molecular Function: metal ion binding; polypeptide N-acetylgalactosaminyltransferase activity; carbohydrate binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; carbohydrate metabolic process; post-translational protein modification