FXYD3 sirna

FXYD3 siRNA (Human)

Cat. Num. AAA8230456

• Gene Names: FXYD3; MAT8; PLML
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FXYD3; FXYD3 siRNA (Human); MAT8; PLML; FXYD domain-containing ion transport regulator 3; Chloride conductance inducer protein Mat-8; Mammary tumor 8 kDa protein; Phospholemman-like; FXYD3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FXYD3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 144

• Applicable Applications for FXYD3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FXYD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FXYD3 sirna

siRNA to inhibit FXYD3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209862809
• NCBI GeneID: 5349
• NCBI Accession #: NP_001129479.1
• NCBI GenBank Nucleotide #: NM_001136007.1
• UniProt Accession #: Q14802; Q13211; Q6IB59; A6NDE0; C9JDU2; F5H174; F8WB34
• Molecular Weight: 6,718 Da
• NCBI Official Full Name: FXYD domain-containing ion transport regulator 3 isoform 3
• NCBI Official Synonym Full Names: FXYD domain containing ion transport regulator 3
• NCBI Official Symbol: FXYD3
• NCBI Official Synonym Symbols: MAT8; PLML
• NCBI Protein Information: FXYD domain-containing ion transport regulator 3
• UniProt Protein Name: FXYD domain-containing ion transport regulator 3
• Protein Family: FXYD domain-containing ion transport regulator
• UniProt Gene Name: FXYD3
• UniProt Synonym Gene Names: MAT8; PLML
• UniProt Entry Name: FXYD3_HUMAN

NCBI Description

This gene belongs to a small family of FXYD-domain containing regulators of Na+/K+ ATPases which share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD, and containing 7 invariant and 6 highly conserved amino acids. This gene encodes a cell membrane protein that may regulate the function of ion-pumps and ion-channels. This gene may also play a role in tumor progression. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]

Uniprot Description

FXYD3: Induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. May be a modulator capable of activating endogenous oocyte channels. Belongs to the FXYD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane

Molecular Function: chloride channel activity; ATPase binding

Biological Process: regulation of catalytic activity; chloride transport; transmembrane transport