FHOD3 sirna

FHOD3 siRNA (Human)

Cat. Num. AAA8212907

• Gene Names: FHOD3; FHOS2; Formactin2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FHOD3; FHOD3 siRNA (Human); FHOS2; KIAA1695; FH1/FH2 domain-containing protein 3; Formactin-2; Formin homolog overexpressed in spleen 2; hFHOS2; FHOD3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FHOD3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1422

• Applicable Applications for FHOD3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FHOD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FHOD3 sirna

siRNA to inhibit FHOD3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 528524522
• NCBI GeneID: 80206
• NCBI Accession #: NP_001268668.1
• NCBI GenBank Nucleotide #: NM_001281739.2
• UniProt Accession #: Q2V2M9; Q642I2; Q6ZRQ7; Q86TF9; Q8N3A5; Q9C0G8; Q9H604; Q9H6G7; A8MQT4; E5F5Q0
• Molecular Weight: 180,080 Da
• NCBI Official Full Name: FH1/FH2 domain-containing protein 3 isoform 2
• NCBI Official Synonym Full Names: formin homology 2 domain containing 3
• NCBI Official Symbol: FHOD3
• NCBI Official Synonym Symbols: FHOS2; Formactin2
• NCBI Protein Information: FH1/FH2 domain-containing protein 3
• UniProt Protein Name: FH1/FH2 domain-containing protein 3
• Protein Family: FH1/FH2 domain-containing protein
• UniProt Gene Name: FHOD3
• UniProt Synonym Gene Names: FHOS2; KIAA1695; hFHOS2
• UniProt Entry Name: FHOD3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the diaphanous-related formins (DRF), and contains multiple domains, including GBD (GTPase-binding domain), DID (diaphanous inhibitory domain), FH1 (formin homology 1), FH2 (formin homology 2), and DAD (diaphanous auto-regulatory domain) domains. This protein is thought to play a role in actin filament polymerization in cardiomyocytes. Mutations in this gene have been associated with dilated cardiomyopathy (DCM), characterized by dilation of the ventricular chamber, leading to impairment of systolic pump function and subsequent heart failure. Increased levels of the protein encoded by this gene have been observed in individuals with hypertrophic cardiomyopathy (HCM). Alternative splicing results in multiple transcript variants encoding different isoforms. A muscle-specific isoform has been shown to possess a casein kinase 2 (CK2) phosphorylation site at the C-terminal end of the FH2 domain. Phosphorylation of this site alters its interaction with sequestosome 1 (SQSTM1), and targets this isoform to myofibrils, while other isoforms form cytoplasmic aggregates. [provided by RefSeq, Aug 2015]

Uniprot Description

FHOD3: Actin-organizing protein that may cause stress fiber formation together with cell elongation. Belongs to the formin homology family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q12

Cellular Component: Z disc; striated muscle thin filament

Molecular Function: protein binding; actin binding

Biological Process: negative regulation of actin filament polymerization; actin filament network formation; cardiac myofibril assembly; sarcomere organization