FES sirna

FES siRNA (Human)

Cat. Num. AAA8214945

• Gene Names: FES; FPS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FES; FES siRNA (Human); FPS; Tyrosine-protein kinase Fes/Fps; Feline sarcoma/Fujinami avian sarcoma oncogene homolog; Proto-oncogene c-Fes; Proto-oncogene c-Fps; p93c-fes; FES sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FES siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 764

• Applicable Applications for FES sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FES gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FES sirna

siRNA to inhibit FES expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 219842230
• NCBI GeneID: 2242
• NCBI Accession #: NP_001137255.1
• NCBI GenBank Nucleotide #: NM_001143783.1
• UniProt Accession #: P07332; Q2VXS7; Q2VXS8; Q2VXT0; Q6GTU5; B2R6E6; B4DUD0; E9PC94; E9PC95
• Molecular Weight: 85,466 Da
• NCBI Official Full Name: tyrosine-protein kinase Fes/Fps isoform 2
• NCBI Official Synonym Full Names: FES proto-oncogene, tyrosine kinase
• NCBI Official Symbol: FES
• NCBI Official Synonym Symbols: FPS
• NCBI Protein Information: tyrosine-protein kinase Fes/Fps
• UniProt Protein Name: Tyrosine-protein kinase Fes/Fps
• Protein Family: Festuclavine dehydrogenase
• UniProt Gene Name: FES
• UniProt Synonym Gene Names: FPS
• UniProt Entry Name: FES_HUMAN

NCBI Description

This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

Fes: a non-receptor tyrosine kinase of the Fer family. Appears to be involved in normal hematopoiesis as well as the development of acute promyelocytic leukemia. Contains one SH2 and one FCH domain. Four LOF point mutations seen in colorectal cancer. Orthologous to v-fes from feline leukemia virus and v-fps from avian transforming virus. Mutant forms are angiogenic. Promotes survival during differentiation, and may act both to promote and inhibit tumors. May be disrupted in the t(15q+;17q-) found in acute promyelocytic leukemia, but the breakpoint does not occur within the gene.

Protein type: Tumor suppressor; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.10.2; TK group; Fer family

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: microtubule cytoskeleton; Golgi apparatus; extrinsic to internal side of plasma membrane; focal adhesion; cytoplasmic membrane-bound vesicle; cytoplasm; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; phosphoinositide binding; ATP binding

Biological Process: regulation of mast cell degranulation; regulation of cell adhesion; epidermal growth factor receptor signaling pathway; axon guidance; cell migration; peptidyl-tyrosine phosphorylation; positive regulation of myeloid cell differentiation; multicellular organismal development; positive regulation of microtubule polymerization; protein amino acid autophosphorylation; chemotaxis; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cell shape; cell proliferation; regulation of cell differentiation; innate immune response