FER sirna

FER siRNA (Human)

Cat. Num. AAA8224917

• Gene Names: FER; TYK3; PPP1R74; p94-Fer
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FER; FER siRNA (Human); TYK3; Tyrosine-protein kinase Fer; Feline encephalitis virus-related kinase FER; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; Proto-oncogene c-Fer; Tyrosine kinase 3; p94-Fer; FER sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FER siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 647

• Applicable Applications for FER sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FER gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FER sirna

siRNA to inhibit FER expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 808392675
• NCBI GeneID: 2241
• NCBI Accession #: NP_001294957.1
• NCBI GenBank Nucleotide #: NM_001308028.1
• UniProt Accession #: P16591; B2RCR4; B4DSQ2; H2FLB8
• Molecular Weight: 51,643 Da
• NCBI Official Full Name: tyrosine-protein kinase Fer isoform b
• NCBI Official Synonym Full Names: fer (fps/fes related) tyrosine kinase
• NCBI Official Symbol: FER
• NCBI Official Synonym Symbols: TYK3; PPP1R74; p94-Fer
• NCBI Protein Information: tyrosine-protein kinase Fer
• UniProt Protein Name: Tyrosine-protein kinase Fer
• Protein Family: Ferritin
• UniProt Gene Name: FER
• UniProt Synonym Gene Names: TYK3
• UniProt Entry Name: FER_HUMAN

NCBI Description

The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]

Uniprot Description

Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.

Protein type: Oncoprotein; Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Fer family

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: microtubule cytoskeleton; extrinsic to internal side of plasma membrane; lamellipodium; cytoplasm; nuclear chromatin; cell cortex; cell junction; nucleus; cytosol; actin cytoskeleton

Molecular Function: protein binding; cadherin binding; protein-tyrosine kinase activity; gamma-catenin binding; non-membrane spanning protein tyrosine kinase activity; actin binding; lipid binding; protein phosphatase 1 binding; protein kinase binding; ATP binding; Rab GTPase binding; epidermal growth factor receptor binding

Biological Process: regulation of mast cell degranulation; regulation of protein amino acid phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; diapedesis; cytokine and chemokine mediated signaling pathway; tyrosine phosphorylation of Stat3 protein; negative regulation of mast cell activation during immune response; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; microtubule cytoskeleton organization and biogenesis; chemotaxis; protein amino acid phosphorylation; regulation of epidermal growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of cell proliferation; cell proliferation; positive regulation of actin filament polymerization; cellular response to insulin stimulus; actin cytoskeleton reorganization; positive regulation of cell proliferation; innate immune response; mitotic cell cycle; cell differentiation; cell adhesion; positive regulation of cell migration