FBXO45 sirna

FBXO45 siRNA (Human)

Cat. Num. AAA828862

• Gene Names: FBXO45; Fbx45
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FBXO45; FBXO45 siRNA (Human); FBX45; F-box/SPRY domain-containing protein 1; F-box only protein 45; hFbxo45; FBXO45 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FBXO45 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 286

• Applicable Applications for FBXO45 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FBXO45 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FBXO45 sirna

siRNA to inhibit FBXO45 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157743247
• NCBI GeneID: 200933
• NCBI Accession #: NP_001099043.1
• NCBI GenBank Nucleotide #: NM_001105573.1
• UniProt Accession #: P0C2W1; A6NF90; D3DXB5
• Molecular Weight: 30,633 Da
• NCBI Official Full Name: F-box/SPRY domain-containing protein 1
• NCBI Official Synonym Full Names: F-box protein 45
• NCBI Official Symbol: FBXO45
• NCBI Official Synonym Symbols: Fbx45
• NCBI Protein Information: F-box/SPRY domain-containing protein 1
• UniProt Protein Name: F-box/SPRY domain-containing protein 1
• UniProt Gene Name: FBXO45
• UniProt Synonym Gene Names: FBX45; hFbxo45
• UniProt Entry Name: FBSP1_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXO45, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (summary by Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Jan 2011]

Uniprot Description

FBXO45: Component of E3 ubiquitin ligase complexes. Required for normal neuromuscular synaptogenesis, axon pathfinding and neuronal migration. Plays a role in the regulation of neurotransmission at mature neurons. May controls synaptic activity by controlling UNC13A via ubiquitin dependent pathway. Specifically recognizes TP73, promoting its ubiquitination and degradation. Belongs to the FBXO45/Fsn family.

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: presynaptic membrane; postsynaptic membrane; postsynaptic density; cell junction

Molecular Function: protein binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; anterior commissure morphogenesis; cerebral cortex tangential migration; protein ubiquitination during ubiquitin-dependent protein catabolic process; neuron migration; protein ubiquitination; cerebral cortex radially oriented cell migration; corticospinal tract morphogenesis; response to DNA damage stimulus