FANCM sirna

FANCM siRNA (Human)

Cat. Num. AAA8229780

• Gene Names: FANCM; FAAP250; KIAA1596
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FANCM; FANCM siRNA (Human); KIAA1596; Fanconi anemia group M protein; Protein FACM; ATP-dependent RNA helicase FANCM; Fanconi anemia-associated polypeptide of 250 kDa; FAAP250; Protein Hef ortholog; FANCM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FANCM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2022

• Applicable Applications for FANCM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FANCM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FANCM sirna

siRNA to inhibit FANCM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 815890934
• NCBI GeneID: 57697
• NCBI Accession #: NP_001295062.1
• NCBI GenBank Nucleotide #: NM_001308133.1
• UniProt Accession #: Q8IYD8; Q3YFH9; Q8N9X6; Q9HCH6; B2RTQ9
• Molecular Weight: 229,281 Da
• NCBI Official Full Name: Fanconi anemia group M protein isoform 2
• NCBI Official Synonym Full Names: Fanconi anemia, complementation group M
• NCBI Official Symbol: FANCM
• NCBI Official Synonym Symbols: FAAP250; KIAA1596
• NCBI Protein Information: Fanconi anemia group M protein
• UniProt Protein Name: Fanconi anemia group M protein
• Protein Family: Fanconi anemia group M protein
• UniProt Gene Name: FANCM
• UniProt Synonym Gene Names: KIAA1596; Protein FACM; FAAP250
• UniProt Entry Name: FANCM_HUMAN

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group M. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

FANCM: ATPase required for FANCD2 ubiquitination, a key reaction in DNA repair. Binds to ssDNA but not to dsDNA. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Defects in FANCM are a cause of Fanconi anemia complementation group M (FANCM). FANCM is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. Belongs to the DEAD box helicase family. DEAH subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 14q21.2

Cellular Component: nucleoplasm

Molecular Function: protein binding; nuclease activity; DNA binding; chromatin binding; helicase activity; ATP binding

Biological Process: resolution of meiotic joint molecules as recombinants; DNA repair; replication fork processing

Disease: Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group M