FAAH2 sirna

FAAH2 siRNA (Human)

Cat. Num. AAA8214789

• Gene Names: FAAH2; AMDD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FAAH2; FAAH2 siRNA (Human); AMDD; Fatty-acid amide hydrolase 2; Amidase domain-containing protein; Anandamide amidohydrolase 2; Oleamide hydrolase 2; FAAH2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FAAH2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 532

• Applicable Applications for FAAH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FAAH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FAAH2 sirna

siRNA to inhibit FAAH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 195972892
• NCBI GeneID: 158584
• NCBI Accession #: NP_777572.2
• NCBI GenBank Nucleotide #: NM_174912.3
• UniProt Accession #: Q6GMR7; Q86VT2; Q96N98
• Molecular Weight: 58,304 Da
• NCBI Official Full Name: fatty-acid amide hydrolase 2
• NCBI Official Synonym Full Names: fatty acid amide hydrolase 2
• NCBI Official Symbol: FAAH2
• NCBI Official Synonym Symbols: AMDD
• NCBI Protein Information: fatty-acid amide hydrolase 2
• UniProt Protein Name: Fatty-acid amide hydrolase 2
• Protein Family: Fatty-acid amide hydrolase
• UniProt Gene Name: FAAH2
• UniProt Synonym Gene Names: AMDD
• UniProt Entry Name: FAAH2_HUMAN

NCBI Description

This gene encodes a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of enzymes. The encoded enzyme is able to catalyze the hydrolysis of a broad range of bioactive lipids, including those from the three main classes of fatty acid amides; N-acylethanolamines, fatty acid primary amides and N-acyl amino acids. This enzyme has a preference for monounsaturated acyl chains as a substrate.[provided by RefSeq, Sep 2009]

Uniprot Description

FAAH2: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes monounsaturated substrate anandamide preferentially as compared to polyunsaturated substrates. Belongs to the amidase family.

Protein type: EC 3.5.1.99; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: integral to membrane

Molecular Function: carbon-nitrogen ligase activity, with glutamine as amido-N-donor; hydrolase activity

Biological Process: metabolic process