EXOC6 sirna

EXOC6 siRNA (Human)

Cat. Num. AAA8226030

• Gene Names: EXOC6; SEC15; EXOC6A; SEC15L; Sec15p; SEC15L1; SEC15L3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EXOC6; EXOC6 siRNA (Human); SEC15A; SEC15L; SEC15L1; Exocyst complex component 6; Exocyst complex component Sec15A; SEC15-like protein 1; EXOC6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EXOC6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 799

• Applicable Applications for EXOC6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EXOC6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EXOC6 sirna

siRNA to inhibit EXOC6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 62243652
• NCBI GeneID: 54536
• NCBI Accession #: NP_001013870.1
• NCBI GenBank Nucleotide #: NM_001013848.2
• UniProt Accession #: Q8TAG9; Q5VXH8; Q9NZ24; E9PHI3
• Molecular Weight: 93,407 Da
• NCBI Official Full Name: exocyst complex component 6 isoform b
• NCBI Official Synonym Full Names: exocyst complex component 6
• NCBI Official Symbol: EXOC6
• NCBI Official Synonym Symbols: SEC15; EXOC6A; SEC15L; Sec15p; SEC15L1; SEC15L3
• NCBI Protein Information: exocyst complex component 6
• UniProt Protein Name: Exocyst complex component 6
• Protein Family: Exocyst complex component
• UniProt Gene Name: EXOC6
• UniProt Synonym Gene Names: SEC15A; SEC15L; SEC15L1
• UniProt Entry Name: EXOC6_HUMAN

NCBI Description

The product of this gene belongs to the SEC15 family. It is highly similar to the protein encoded by Saccharomyces cerevisiae SEC15 gene. This protein is essential for vesicular traffic from the Golgi apparatus to the cell surface in yeast. It is one of the components of a multiprotein complex required for exocytosis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

EXOC6: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Together with RAB11A, RAB3IP, RAB8A, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Belongs to the SEC15 family.

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: exocyst; plasma membrane; cytosol

Biological Process: protein transport; cellular protein metabolic process; organelle organization and biogenesis; erythrocyte differentiation; vesicle docking during exocytosis