ESRP2 sirna

ESRP2 siRNA (Human)

Cat. Num. AAA8203280

• Gene Names: ESRP2; RBM35B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ESRP2; ESRP2 siRNA (Human); RBM35B; Epithelial splicing regulatory protein 2; RNA-binding motif protein 35B; RNA-binding protein 35B; ESRP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ESRP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 717

• Applicable Applications for ESRP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ESRP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ESRP2 sirna

siRNA to inhibit ESRP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 45935393
• NCBI GeneID: 80004
• NCBI Accession #: NP_079215.2
• NCBI GenBank Nucleotide #: NM_024939.2
• UniProt Accession #: Q9H6T0; Q8N6H8; Q8WZ15; Q9H6I4
• Molecular Weight: 77,364 Da
• NCBI Official Full Name: epithelial splicing regulatory protein 2
• NCBI Official Synonym Full Names: epithelial splicing regulatory protein 2
• NCBI Official Symbol: ESRP2
• NCBI Official Synonym Symbols: RBM35B
• NCBI Protein Information: epithelial splicing regulatory protein 2
• UniProt Protein Name: Epithelial splicing regulatory protein 2
• Protein Family: Epithelial splicing regulatory protein
• UniProt Gene Name: ESRP2
• UniProt Synonym Gene Names: RBM35B
• UniProt Entry Name: ESRP2_HUMAN

NCBI Description

ESPR2 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009]

Uniprot Description

RBM35B: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: mRNA binding; nucleotide binding

Biological Process: regulation of RNA splicing; RNA splicing; mRNA processing