ERVW-1 sirna

ERVW-1 siRNA (Human)

Cat. Num. AAA8228264

• Gene Names: ERVW-1; ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERVW-1; ERVW-1 siRNA (Human); ERVWE1; HERV-W_7q21.2 provirus ancestral Env polyprotein; Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; Syncytin; Syncytin-1; ERVW-1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ERVW-1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 538

• Applicable Applications for ERVW-1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERVW-1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERVW-1 sirna

siRNA to inhibit ERVW-1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 195963433
• NCBI GeneID: 30816
• NCBI Accession #: NP_001124397.1
• NCBI GenBank Nucleotide #: NM_001130925.1
• UniProt Accession #: Q9UQF0; O95244; O95245; Q8NHY7; Q9NRZ2; Q9NZG3; B2RPD4
• Molecular Weight: 59,866 Da
• NCBI Official Full Name: syncytin-1
• NCBI Official Synonym Full Names: endogenous retrovirus group W, member 1
• NCBI Official Symbol: ERVW-1
• NCBI Official Synonym Symbols: ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV
• NCBI Protein Information: syncytin-1
• UniProt Protein Name: Syncytin-1
• Protein Family: Syncytin
• UniProt Gene Name: ERVW-1
• UniProt Synonym Gene Names: ERVWE1; SU; TM
• UniProt Entry Name: SYCY1_HUMAN

NCBI Description

Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

ERVWE1: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. Belongs to the gamma type-C retroviral envelope protein family. HERV class-I W env subfamily.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 7q21.2

Cellular Component: plasma membrane; integral to membrane; viral envelope

Biological Process: anatomical structure morphogenesis; syncytium formation