ERG sirna

ERG siRNA (Human)

Cat. Num. AAA8224830

• Gene Names: ERG; p55; erg-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERG; ERG siRNA (Human); Transcriptional regulator ERG; Transforming protein ERG; ERG sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ERG siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 486

• Applicable Applications for ERG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERG sirna

siRNA to inhibit ERG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209954797
• NCBI GeneID: 2078
• NCBI Accession #: NP_001129626.1
• NCBI GenBank Nucleotide #: NM_001136154.1
• UniProt Accession #: P11308; Q16113; Q6XXX4; Q6XXX5; Q8IXK9; B4DTW5; B4E0T4
• Molecular Weight: 36,541 Da
• NCBI Official Full Name: transcriptional regulator ERG isoform 3
• NCBI Official Synonym Full Names: v-ets avian erythroblastosis virus E26 oncogene homolog
• NCBI Official Symbol: ERG
• NCBI Official Synonym Symbols: p55; erg-3
• NCBI Protein Information: transcriptional regulator ERG
• UniProt Protein Name: Transcriptional regulator ERG
• Protein Family: Ergosterol biosynthetic protein
• UniProt Gene Name: ERG
• UniProt Entry Name: ERG_HUMAN

NCBI Description

This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Apr 2014]

Uniprot Description

ERG: Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. Defects in ERG are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1. Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4. Belongs to the ETS family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; cell proliferation; multicellular organismal development; cell differentiation; signal transduction; protein amino acid phosphorylation