ERCC2 sirna

ERCC2 siRNA (Human)

Cat. Num. AAA8220914

• Gene Names: ERCC2; EM9; TTD; XPD; TTD1; COFS2; TFIIH
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERCC2; ERCC2 siRNA (Human); XPD; XPDC; TFIIH basal transcription factor complex helicase XPD subunit; Basic transcription factor 2 80 kDa subunit; BTF2 p80; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH 80 kDa subunit; TFIIH p80; Xeroderma pigmentosum group D-complementing protein; ERCC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ERCC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 405

• Applicable Applications for ERCC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERCC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERCC2 sirna

siRNA to inhibit ERCC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15834617
• NCBI GeneID: 2068
• NCBI Accession #: NP_000391.1
• NCBI GenBank Nucleotide #: NM_000400.3
• UniProt Accession #: P18074; Q2TB78; Q2YDY2; Q7KZU6; Q8N721
• Molecular Weight: 46,274 Da
• NCBI Official Full Name: TFIIH basal transcription factor complex helicase XPD subunit isoform 1
• NCBI Official Synonym Full Names: excision repair cross-complementation group 2
• NCBI Official Symbol: ERCC2
• NCBI Official Synonym Symbols: EM9; TTD; XPD; TTD1; COFS2; TFIIH
• NCBI Protein Information: TFIIH basal transcription factor complex helicase XPD subunit
• UniProt Protein Name: TFIIH basal transcription factor complex helicase XPD subunit
• Protein Family: TFIIH basal transcription factor complex helicase
• UniProt Gene Name: ERCC2
• UniProt Synonym Gene Names: XPD; XPDC; BTF2 p80; TFIIH 80 kDa subunit; TFIIH p80
• UniProt Entry Name: ERCC2_HUMAN

NCBI Description

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

ERCC2: ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. Might also have a role in aging process and could play a causative role in the generation of skin cancers. Belongs to the helicase family. RAD3/XPD subfamily. One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Interacts with Epstein-Barr virus EBNA2.

Protein type: EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; holo TFIIH complex; cytoplasm; spindle; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: protein C-terminus binding; RNA polymerase subunit kinase activity; ATP-dependent DNA helicase activity; DNA-dependent ATPase activity; protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; protein N-terminus binding; 5'-3' DNA helicase activity; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; extracellular matrix organization and biogenesis; erythrocyte maturation; viral reproduction; apoptosis; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; protein amino acid phosphorylation; bone mineralization; embryonic cleavage; chromosome segregation; post-embryonic development; mRNA capping; UV protection; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; aging; positive regulation of DNA binding; transcription initiation from RNA polymerase II promoter; in utero embryonic development; multicellular organism growth; nucleotide-excision repair, DNA incision; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; hair cell differentiation; DNA repair; hair follicle maturation; termination of RNA polymerase I transcription; DNA duplex unwinding; cell proliferation; myelin formation in the central nervous system; spinal cord development; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; gene expression; response to oxidative stress; transcription initiation from RNA polymerase I promoter

Disease: Cerebrooculofacioskeletal Syndrome 2; Xeroderma Pigmentosum, Complementation Group D; Trichothiodystrophy, Photosensitive