ERCC1 sirna

ERCC1 siRNA (Human)

Cat. Num. AAA8200396

• Gene Names: ERCC1; UV20; COFS4; RAD10
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERCC1; ERCC1 siRNA (Human); DNA excision repair protein ERCC-1; ERCC1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ERCC1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 273

• Applicable Applications for ERCC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERCC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERCC1 sirna

siRNA to inhibit ERCC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 260593725
• NCBI GeneID: 2067
• NCBI Accession #: NP_001159521.1
• NCBI GenBank Nucleotide #: NM_001166049.1
• UniProt Accession #: P07992; Q7Z7F5; Q96S40; B2RC01; B3KRR0
• Molecular Weight: 25,211 Da
• NCBI Official Full Name: DNA excision repair protein ERCC-1 isoform 3
• NCBI Official Synonym Full Names: excision repair cross-complementation group 1
• NCBI Official Symbol: ERCC1
• NCBI Official Synonym Symbols: UV20; COFS4; RAD10
• NCBI Protein Information: DNA excision repair protein ERCC-1
• UniProt Protein Name: DNA excision repair protein ERCC-1
• Protein Family: DNA excision repair protein
• UniProt Gene Name: ERCC1
• UniProt Entry Name: ERCC1_HUMAN

NCBI Description

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Uniprot Description

ERCC1: a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA excision repair. Belongs to the ERCC1/RAD10/SWI10 family. Heterodimer composed of ERCC1 and XPF/ERRC4. Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4, a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. Low levels of this protein are associated with increased sensitivity to cisplatin. The overall survival of non-small cell lung cancer patients with single-nucleotide polymorphisms at codon 118 receiving platinum-based chemotherapy was significantly improved.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; nucleotide-excision repair complex; transcription factor TFIID complex; nuclear chromosome, telomeric region; cytoplasm

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; structure-specific DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; TATA-binding protein binding; damaged DNA binding; single-stranded DNA binding

Biological Process: oogenesis; chromosome organization and biogenesis; DNA recombination; negative regulation of telomere maintenance; UV protection; post-embryonic hemopoiesis; double-strand break repair; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA incision, 5'-to lesion; response to nutrient; response to X-ray; mitotic recombination; multicellular organism growth; male gonad development; isotype switching; pyrimidine dimer repair via nucleotide-excision repair; multicellular organismal aging; replicative cell aging; DNA repair; DNA catabolic process, endonucleolytic; syncytium formation; response to sucrose stimulus; nucleotide-excision repair, DNA incision, 3'-to lesion; cell proliferation; nucleotide-excision repair; embryonic organ development; spermatogenesis; response to oxidative stress

Disease: Cerebrooculofacioskeletal Syndrome 4