EPPIN sirna

EPPIN siRNA (Human)

Cat. Num. AAA8239861

• Gene Names: EPPIN-WFDC6; SPINLW1-WFDC6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPPIN; EPPIN siRNA (Human); SPINLW1; WAP7; WFDC7; Eppin; Cancer/testis antigen 71; CT71; Epididymal protease inhibitor; Protease inhibitor WAP7; Serine protease inhibitor-like with Kunitz and WAP domains 1; WAP four-disulfide core domain protein 7; EPPIN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EPPIN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 179

• Applicable Applications for EPPIN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EPPIN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPPIN sirna

siRNA to inhibit EPPIN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 312176446
• NCBI GeneID: 100526773
• NCBI Accession #: NP_001185915.1
• NCBI GenBank Nucleotide #: NM_001198986.1
• UniProt Accession #: O95925; Q86TP9; Q96SD7; Q9HD30; A6PVD6
• Molecular Weight: 20,486 Da
• NCBI Official Full Name: SPINLW1-WFDC6 fusion protein
• NCBI Official Synonym Full Names: EPPIN-WFDC6 readthrough
• NCBI Official Symbol: EPPIN-WFDC6
• NCBI Official Synonym Symbols: SPINLW1-WFDC6
• NCBI Protein Information: SPINLW1-WFDC6 fusion protein
• UniProt Protein Name: Eppin
• Protein Family: Eppin
• UniProt Gene Name: EPPIN
• UniProt Synonym Gene Names: SPINLW1; WAP7; WFDC7; CT71
• UniProt Entry Name: EPPI_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring SPINLW1 (serine peptidase inhibitor-like, with Kunitz and WAP domains 1) and WFDC6 (WAP four-disulfide core domain 6) genes. The read-through transcript encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq, Nov 2010]

Uniprot Description

Spinlw1: Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell surface; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding

Biological Process: defense response to bacterium; negative regulation of peptidase activity