ENTPD3 sirna

ENTPD3 siRNA (Human)

Cat. Num. AAA827388

• Gene Names: ENTPD3; HB6; CD39L3; NTPDase-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ENTPD3; ENTPD3 siRNA (Human); CD39L3; Ectonucleoside triphosphate diphosphohydrolase 3; NTPDase 3; CD39 antigen-like 3; Ecto-ATP diphosphohydrolase 3; Ecto-ATPDase 3; Ecto-ATPase 3; Ecto-apyrase 3; HB6; ENTPD3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ENTPD3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 529

• Applicable Applications for ENTPD3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ENTPD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ENTPD3 sirna

siRNA to inhibit ENTPD3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 166197702
• NCBI GeneID: 956
• NCBI Accession #: NP_001239.2
• NCBI GenBank Nucleotide #: NM_001248.3
• UniProt Accession #: O75355; O60495; Q8N6K2; B2R8D0; G5E9N0
• Molecular Weight: 50,762 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 3 isoform 1
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 3
• NCBI Official Symbol: ENTPD3
• NCBI Official Synonym Symbols: HB6; CD39L3; NTPDase-3
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 3
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 3
• Protein Family: Ectonucleoside triphosphate diphosphohydrolase
• UniProt Gene Name: ENTPD3
• UniProt Synonym Gene Names: CD39L3; NTPDase 3; Ecto-ATPDase 3; Ecto-ATPase 3
• UniProt Entry Name: ENTP3_HUMAN

NCBI Description

This gene encodes a plasma membrane-bound divalent cation-dependent E-type nucleotidase. The encoded protein is involved in the regulation of extracellular levels of ATP by hydrolysis of it and other nucleotides. Multiple transcript variants have been described. [provided by RefSeq, May 2014]

Uniprot Description

ENTPD3: Has a threefold preference for the hydrolysis of ATP over ADP. Belongs to the GDA1/CD39 NTPase family.

Protein type: Nucleotide Metabolism - pyrimidine; EC 3.6.1.5; Hydrolase; Membrane protein, multi-pass; Membrane protein, integral; Nucleotide Metabolism - purine; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: nucleoside-diphosphatase activity; nucleoside-triphosphatase activity; ATP binding

Biological Process: nucleoside diphosphate catabolic process; nucleoside triphosphate catabolic process