ELOVL5 sirna

ELOVL5 siRNA (Human)

Cat. Num. AAA8207354

• Gene Names: ELOVL5; HELO1; SCA38; dJ483K16.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ELOVL5; ELOVL5 siRNA (Human); ELOVL2; Elongation of very long chain fatty acids protein 5; 3-keto acyl-CoA synthase ELOVL5; ELOVL fatty acid elongase 5; ELOVL FA elongase 5; Fatty acid elongase 1; hELO1; Very-long-chain 3-oxoacyl-CoA synthase 5; ELOVL5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ELOVL5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 88

• Applicable Applications for ELOVL5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ELOVL5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ELOVL5 sirna

siRNA to inhibit ELOVL5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 338827646
• NCBI GeneID: 60481
• NCBI Accession #: NP_001229757.1
• NCBI GenBank Nucleotide #: NM_001242828.1
• UniProt Accession #: Q9NYP7; Q59EL3; Q5TGH5; Q6NXE7; Q7L2S5; Q8NCG4; Q9UI22; B4DZJ2; F6SH78
• Molecular Weight: 9,580 Da
• NCBI Official Full Name: elongation of very long chain fatty acids protein 5 isoform 2
• NCBI Official Synonym Full Names: ELOVL fatty acid elongase 5
• NCBI Official Symbol: ELOVL5
• NCBI Official Synonym Symbols: HELO1; SCA38; dJ483K16.1
• NCBI Protein Information: elongation of very long chain fatty acids protein 5
• UniProt Protein Name: Elongation of very long chain fatty acids protein 5
• UniProt Gene Name: ELOVL5
• UniProt Synonym Gene Names: ELOVL2; ELOVL FA elongase 5; hELO1
• UniProt Entry Name: ELOV5_HUMAN

NCBI Description

This gene belongs to the ELO family. It is highly expressed in the adrenal gland and testis, and encodes a multi-pass membrane protein that is localized in the endoplasmic reticulum. This protein is involved in the elongation of long-chain polyunsaturated fatty acids. Mutations in this gene have been associated with spinocerebellar ataxia-38 (SCA38). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

ELOVL5: Condensing enzyme that catalyzes the synthesis of monounsaturated and of polyunsaturated very long chain fatty acids Acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. Belongs to the ELO family.

Protein type: EC 2.3.1.199; Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - unsaturated fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 6p12.1

Cellular Component: endoplasmic reticulum membrane; membrane; cell soma; endoplasmic reticulum; dendrite; integral to membrane

Molecular Function: protein binding; fatty acid elongase activity

Biological Process: unsaturated fatty acid metabolic process; very-long-chain fatty acid biosynthetic process; linoleic acid metabolic process; unsaturated fatty acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process

Disease: Spinocerebellar Ataxia 38