EIF5 sirna

EIF5 siRNA (Human)

Cat. Num. AAA8200910

• Gene Names: EIF5; EIF-5; EIF-5A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF5; EIF5 siRNA (Human); Eukaryotic translation initiation factor 5; eIF-5; EIF5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EIF5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 431

• Applicable Applications for EIF5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EIF5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF5 sirna

siRNA to inhibit EIF5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21361337
• NCBI GeneID: 1983
• NCBI Accession #: NP_001960.2
• NCBI GenBank Nucleotide #: NM_001969.4
• UniProt Accession #: P55010; Q53XB3; Q9H5N2; Q9UG48
• Molecular Weight: 49,223 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 5
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 5
• NCBI Official Symbol: EIF5
• NCBI Official Synonym Symbols: EIF-5; EIF-5A
• NCBI Protein Information: eukaryotic translation initiation factor 5
• UniProt Protein Name: Eukaryotic translation initiation factor 5
• UniProt Gene Name: EIF5
• UniProt Synonym Gene Names: eIF-5
• UniProt Entry Name: IF5_HUMAN

NCBI Description

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]

Uniprot Description

EIF5: eukaryotic translation initiation factor 5. Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: GTPase activity; GTP binding; translation factor activity, nucleic acid binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; regulation of translational initiation