EGLN2 sirna

EGLN2 siRNA (Human)

Cat. Num. AAA8237009

• Gene Names: EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EGLN2; EGLN2 siRNA (Human); EIT6; Egl nine homolog 2; Estrogen-induced tag 6; HPH-3; Hypoxia-inducible factor prolyl hydroxylase 1; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; Prolyl hydroxylase domain-containing protein 1; PHD1; EGLN2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EGLN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 374

• Applicable Applications for EGLN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EGLN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EGLN2 sirna

siRNA to inhibit EGLN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16604260
• NCBI GeneID: 112398
• NCBI Accession #: NP_444274.1
• NCBI GenBank Nucleotide #: NM_053046.3
• UniProt Accession #: Q96KS0; Q8WWY4; Q9BV14; A8K5S0
• Molecular Weight: 40,168 Da
• NCBI Official Full Name: egl nine homolog 2
• NCBI Official Synonym Full Names: egl-9 family hypoxia-inducible factor 2
• NCBI Official Symbol: EGLN2
• NCBI Official Synonym Symbols: EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
• NCBI Protein Information: egl nine homolog 2
• UniProt Protein Name: Egl nine homolog 2
• Protein Family: Egl nine
• UniProt Gene Name: EGLN2
• UniProt Synonym Gene Names: EIT6; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; PHD1
• UniProt Entry Name: EGLN2_HUMAN

NCBI Description

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

Uniprot Description

EGLN2: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 1.14.11.29; Oxidoreductase

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: protein binding; oxygen sensor activity; L-ascorbic acid binding; ferrous iron binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity

Biological Process: estrogen receptor signaling pathway; cell redox homeostasis; regulation of neuron apoptosis; response to hypoxia; positive regulation of protein catabolic process; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; regulation of cell growth