EFNA4 sirna

EFNA4 siRNA (Human)

Cat. Num. AAA8211732

• Gene Names: EFNA4; EFL4; EPLG4; LERK4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EFNA4; EFNA4 siRNA (Human); EPLG4; LERK4; Ephrin-A4; EPH-related receptor tyrosine kinase ligand 4; LERK-4; EFNA4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: EFNA4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 201

• Applicable Applications for EFNA4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EFNA4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EFNA4 sirna

siRNA to inhibit EFNA4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4885197
• NCBI GeneID: 1945
• NCBI Accession #: NP_005218.1
• NCBI GenBank Nucleotide #: NM_005227.2
• UniProt Accession #: P52798; O95457; Q5SR71; Q6FI57; C9JHJ8; G3XAK2
• Molecular Weight: 22,584 Da
• NCBI Official Full Name: ephrin-A4 isoform a
• NCBI Official Synonym Full Names: ephrin-A4
• NCBI Official Symbol: EFNA4
• NCBI Official Synonym Symbols: EFL4; EPLG4; LERK4
• NCBI Protein Information: ephrin-A4
• UniProt Protein Name: Ephrin-A4
• Protein Family: Ephrin
• UniProt Gene Name: EFNA4
• UniProt Synonym Gene Names: EPLG4; LERK4; LERK-4
• UniProt Entry Name: EFNA4_HUMAN

NCBI Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin. Three transcript variants that encode distinct proteins have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA4: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: protein binding; transmembrane-ephrin receptor activity; ephrin receptor binding

Biological Process: axon guidance; cell-cell signaling; ephrin receptor signaling pathway; osteoclast differentiation; bone remodeling