DYM sirna

DYM siRNA (Human)

Cat. Num. AAA8214094

• Gene Names: DYM; DMC; SMC
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DYM; DYM siRNA (Human); Dymeclin; Dyggve-Melchior-Clausen syndrome protein; DYM sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DYM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 669

• Applicable Applications for DYM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DYM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DYM sirna

siRNA to inhibit DYM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157779137
• NCBI GeneID: 54808
• NCBI Accession #: NP_060123.3
• NCBI GenBank Nucleotide #: NM_017653.3
• UniProt Accession #: Q7RTS9; Q3ZTS8; Q6P2P5; Q8N2M0; Q9BVE9; Q9NPU7; A8K5I8; B2RCF9; B4DKI7
• Molecular Weight: 54,425 Da
• NCBI Official Full Name: dymeclin
• NCBI Official Synonym Full Names: dymeclin
• NCBI Official Symbol: DYM
• NCBI Official Synonym Symbols: DMC; SMC
• NCBI Protein Information: dymeclin
• UniProt Protein Name: Dymeclin
• Protein Family: Dymeclin
• UniProt Gene Name: DYM
• UniProt Entry Name: DYM_HUMAN

NCBI Description

This gene encodes a protein which is necessary for normal skeletal development and brain function. Mutations in this gene are associated with two types of recessive osteochondrodysplasia, Dyggve-Melchior-Clausen (DMC) dysplasia and Smith-McCort (SMC) dysplasia, which involve both skeletal defects and mental retardation. [provided by RefSeq, Jul 2008]

Uniprot Description

DYM: Necessary for correct organization of Golgi apparatus. Involved in bone development. Defects in DYM are the cause of Dyggve-Melchior-Clausen syndrome (DMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation. Electron microscopic study of cutaneous cells of affected patients shows dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles. DMC is progressive. Defects in DYM are the cause of Smith-McCort dysplasia (SMC). SMC is a rare autosomal recessive osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest. The radiographic phenotype includes platyspondyly, generalized abnormalities of the epiphyses and metaphyses, and a distinctive lacy appearance of the iliac crest, features identical to those of Dyggve-Melchior-Clausen syndrome. Belongs to the dymeclin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; cytoplasm

Molecular Function: protein binding; enzyme binding

Biological Process: Golgi organization and biogenesis

Disease: Dyggve-melchior-clausen Disease; Smith-mccort Dysplasia 1