DUOX2 sirna

DUOX2 siRNA (Human)

Cat. Num. AAA829327

• Gene Names: DUOX2; TDH6; LNOX2; THOX2; NOXEF2; P138-TOX
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DUOX2; DUOX2 siRNA (Human); LNOX2; THOX2; Dual oxidase 2; Large NOX 2; Long NOX 2; NADH/NADPH thyroid oxidase p138-tox; NADPH oxidase/peroxidase DUOX2; NADPH thyroid oxidase 2; Thyroid oxidase 2; p138 thyroid oxidase; DUOX2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DUOX2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1548

• Applicable Applications for DUOX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DUOX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DUOX2 sirna

siRNA to inhibit DUOX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 132566532
• NCBI GeneID: 50506
• NCBI Accession #: NP_054799.4
• NCBI GenBank Nucleotide #: NM_014080.4
• UniProt Accession #: Q9NRD8; Q9NR02; Q9UHF9; A8MQ13; D2XI64
• Molecular Weight: 175,364 Da
• NCBI Official Full Name: dual oxidase 2
• NCBI Official Synonym Full Names: dual oxidase 2
• NCBI Official Symbol: DUOX2
• NCBI Official Synonym Symbols: TDH6; LNOX2; THOX2; NOXEF2; P138-TOX
• NCBI Protein Information: dual oxidase 2
• UniProt Protein Name: Dual oxidase 2
• Protein Family: Dual oxidase
• UniProt Gene Name: DUOX2
• UniProt Synonym Gene Names: LNOX2; THOX2
• UniProt Entry Name: DUOX2_HUMAN

NCBI Description

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX1. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. [provided by RefSeq, Jul 2008]

Uniprot Description

DUOX2: Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain. Defects in DUOX2 are a cause of thyroid dyshormonogenesis 6 (TDH6). A disorder due to a defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.6.3.1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: apical plasma membrane; integral to membrane

Molecular Function: peroxidase activity; NAD(P)H oxidase activity; heme binding; calcium ion binding

Biological Process: response to cAMP; cytokine and chemokine mediated signaling pathway; multicellular organism growth; response to virus; thyroid hormone generation; bone mineralization; adenohypophysis morphogenesis; cuticle development; fertilization; hydrogen peroxide catabolic process; thyroid gland development; hormone biosynthetic process; response to oxidative stress; inner ear development

Disease: Thyroid Dyshormonogenesis 6