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DTX3L sirna

DTX3L siRNA (Human)

Gene Names
DTX3L; BBAP
Reactivity
Human
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
DTX3L; DTX3L siRNA (Human); BBAP; E3 ubiquitin-protein ligase DTX3L; B-lymphoma- and BAL-associated protein; Protein deltex-3-like; Rhysin-2; Rhysin2; DTX3L sirna
Ordering
 
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For Research Use Only!
Host
Synthetic
Reactivity
Human
Specificity
DTX3L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
740
Applicable Applications for DTX3L sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DTX3L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for DTX3L sirna
siRNA to inhibit DTX3L expression using RNA interference

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,792 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase DTX3L
NCBI Official Synonym Full Names
deltex 3 like, E3 ubiquitin ligase
NCBI Official Symbol
DTX3L
NCBI Official Synonym Symbols
BBAP
NCBI Protein Information
E3 ubiquitin-protein ligase DTX3L
UniProt Protein Name
E3 ubiquitin-protein ligase DTX3L
UniProt Gene Name
DTX3L
UniProt Synonym Gene Names
BBAP; Rhysin2
UniProt Entry Name
DTX3L_HUMAN

NCBI Description

DTX3L functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009]

Uniprot Description

DTX3L: Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post- translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation. Belongs to the Deltex family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q21.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; histone binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: histone monoubiquitination; double-strand break repair; response to DNA damage stimulus

Research Articles on DTX3L

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Product Notes

The DTX3L dtx3l (Catalog #AAA8204018) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The DTX3L siRNA (Human) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DTX3L can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the DTX3L dtx3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DTX3L, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.
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