DDX23 sirna

DDX23 siRNA (Human)

Cat. Num. AAA8213729

• Gene Names: DDX23; prp28; PRPF28; U5-100K; SNRNP100; U5-100KD
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DDX23; DDX23 siRNA (Human); Probable ATP-dependent RNA helicase DDX23; 100 kDa U5 snRNP-specific protein; DEAD box protein 23; PRP28 homolog; U5-100kD; DDX23 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DDX23 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 820

• Applicable Applications for DDX23 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DDX23 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DDX23 sirna

siRNA to inhibit DDX23 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41327771
• NCBI GeneID: 9416
• NCBI Accession #: NP_004809.2
• NCBI GenBank Nucleotide #: NM_004818.2
• UniProt Accession #: Q9BUQ8; O43188; B2R600; B4DH15
• Molecular Weight: 13,401 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX23
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 23
• NCBI Official Symbol: DDX23
• NCBI Official Synonym Symbols: prp28; PRPF28; U5-100K; SNRNP100; U5-100KD
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX23
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX23
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX23
• UniProt Entry Name: DDX23_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX23: Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation. Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.

Protein type: RNA-binding; Helicase; Spliceosome; EC 3.6.4.13; RNA splicing; RNA processing

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: nucleoplasm; mitochondrion; snRNP U5; plasma membrane; nucleolus; nucleus

Molecular Function: protein binding; ATP-dependent helicase activity; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; cis assembly of pre-catalytic spliceosome; RNA splicing; gene expression