DCXR sirna

DCXR siRNA (Human)

Cat. Num. AAA8207962

• Gene Names: DCXR; XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DCXR; DCXR siRNA (Human); L-xylulose reductase; XR; Carbonyl reductase II; Dicarbonyl/L-xylulose reductase; Kidney dicarbonyl reductase; kiDCR; Sperm surface protein P34H; DCXR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DCXR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 244

• Applicable Applications for DCXR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DCXR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DCXR sirna

siRNA to inhibit DCXR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 304571975
• NCBI GeneID: 51181
• NCBI Accession #: NP_001182147.1
• NCBI GenBank Nucleotide #: NM_001195218.1
• UniProt Accession #: Q7Z4W1; Q9BTZ3; Q9UHY9
• Molecular Weight: 25,913 Da
• NCBI Official Full Name: L-xylulose reductase isoform 2
• NCBI Official Synonym Full Names: dicarbonyl/L-xylulose reductase
• NCBI Official Symbol: DCXR
• NCBI Official Synonym Symbols: XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• NCBI Protein Information: L-xylulose reductase
• UniProt Protein Name: L-xylulose reductase
• UniProt Gene Name: DCXR
• UniProt Synonym Gene Names: SDR20C1; XR; kiDCR
• UniProt Entry Name: DCXR_HUMAN

NCBI Description

The protein encoded by this gene acts as a homotetramer to catalyze diacetyl reductase and L-xylulose reductase reactions. The encoded protein may play a role in the uronate cycle of glucose metabolism and in the cellular osmoregulation in the proximal renal tubules. Defects in this gene are a cause of pentosuria. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

DCXR: Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L- xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.10; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: microvillus; membrane; cytoplasmic microtubule; nucleus; brush border

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; L-xylulose reductase activity

Biological Process: glucose metabolic process; xylulose metabolic process; D-xylose metabolic process; NADP metabolic process; protein homotetramerization

Disease: Pentosuria