CYBB sirna

CYBB siRNA (Human)

Cat. Num. AAA8207793

• Gene Names: CYBB; CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYBB; CYBB siRNA (Human); NOX2; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; Superoxide-generating NADPH oxidase heavy chain subunit; gp91-1; gp91-phox; p22 phagocyte B-cytochrome; CYBB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CYBB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 570

• Applicable Applications for CYBB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CYBB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYBB sirna

siRNA to inhibit CYBB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6996021
• NCBI GeneID: 1536
• NCBI Accession #: NP_000388.2
• NCBI GenBank Nucleotide #: NM_000397.3
• UniProt Accession #: P04839; Q2PP16; A8K138
• Molecular Weight: 65,336 Da
• NCBI Official Full Name: cytochrome b-245 heavy chain
• NCBI Official Synonym Full Names: cytochrome b-245, beta polypeptide
• NCBI Official Symbol: CYBB
• NCBI Official Synonym Symbols: CGD; NOX2; IMD34; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• NCBI Protein Information: cytochrome b-245 heavy chain
• UniProt Protein Name: Cytochrome b-245 heavy chain
• Protein Family: Cytochrome
• UniProt Gene Name: CYBB
• UniProt Synonym Gene Names: NOX2; Cytochrome b558 subunit beta
• UniProt Entry Name: CY24B_HUMAN

NCBI Description

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

Uniprot Description

CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections.

Protein type: Oxidoreductase; Membrane protein, multi-pass; Mitochondrial; Membrane protein, integral; EC 1.-.-.-

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; mitochondrion; cell soma; rough endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; NADPH oxidase complex

Molecular Function: protein binding; FAD binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; voltage-gated ion channel activity

Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; ion transport; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; response to nutrient; hydrogen peroxide biosynthetic process

Disease: Immunodeficiency 34; Granulomatous Disease, Chronic, X-linked