CTNNBIP1 sirna

CTNNBIP1 siRNA (Human)

Cat. Num. AAA8215185

• Gene Names: CTNNBIP1; ICAT
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTNNBIP1; CTNNBIP1 siRNA (Human); ICAT; Beta-catenin-interacting protein 1; Inhibitor of beta-catenin and Tcf-4; CTNNBIP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CTNNBIP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 81

• Applicable Applications for CTNNBIP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTNNBIP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTNNBIP1 sirna

siRNA to inhibit CTNNBIP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 59889555
• NCBI GeneID: 56998
• NCBI Accession #: NP_001012329.1
• NCBI GenBank Nucleotide #: NM_001012329.1
• UniProt Accession #: Q9NSA3; Q5T4V2
• Molecular Weight: 9,170 Da
• NCBI Official Full Name: beta-catenin-interacting protein 1
• NCBI Official Synonym Full Names: catenin, beta interacting protein 1
• NCBI Official Symbol: CTNNBIP1
• NCBI Official Synonym Symbols: ICAT
• NCBI Protein Information: beta-catenin-interacting protein 1
• UniProt Protein Name: Beta-catenin-interacting protein 1
• Protein Family: Beta-catenin-interacting protein
• UniProt Gene Name: CTNNBIP1
• UniProt Synonym Gene Names: ICAT
• UniProt Entry Name: CNBP1_HUMAN

NCBI Description

The protein encoded by this gene binds CTNNB1 and prevents interaction between CTNNB1 and TCF family members. The encoded protein is a negative regulator of the Wnt signaling pathway. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTNNBIP1: Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway. Belongs to the CTNNBIP1 family.

Protein type: Inhibitor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol; beta-catenin destruction complex

Molecular Function: protein binding; beta-catenin binding

Biological Process: positive regulation of monocyte differentiation; anterior/posterior pattern formation; positive regulation of osteoblast differentiation; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; negative regulation of smooth muscle cell proliferation; ureteric bud branching; negative regulation of transcription factor activity; negative regulation of protein binding; regulation of vascular permeability during acute inflammatory response; negative regulation of DNA binding