CTNNA1 sirna

CTNNA1 siRNA (Human)

Cat. Num. AAA8231983

• Gene Names: CTNNA1; CAP102
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTNNA1; CTNNA1 siRNA (Human); Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; CTNNA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CTNNA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 841

• Applicable Applications for CTNNA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTNNA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTNNA1 sirna

siRNA to inhibit CTNNA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 594140506
• NCBI GeneID: 1495
• NCBI Accession #: NP_001277236.1
• NCBI GenBank Nucleotide #: NM_001290307.1
• UniProt Accession #: P35221; Q12795; Q8N1C0
• Molecular Weight: 59,550 Da
• NCBI Official Full Name: catenin alpha-1 isoform 2
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), alpha 1, 102kDa
• NCBI Official Symbol: CTNNA1
• NCBI Official Synonym Symbols: CAP102
• NCBI Protein Information: catenin alpha-1
• UniProt Protein Name: Catenin alpha-1
• Protein Family: Catenin
• UniProt Gene Name: CTNNA1
• UniProt Entry Name: CTNA1_HUMAN

Uniprot Description

CTNNA1: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation. Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds MLLT4 and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma- catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F- actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1. Expressed ubiquitously in normal tissues. Belongs to the vinculin/alpha-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: focal adhesion; lamellipodium; plasma membrane; acrosome; zonula adherens; catenin complex; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; cadherin binding; gamma-catenin binding; beta-catenin binding; structural molecule activity; vinculin binding

Biological Process: intercellular junction assembly and maintenance; apical junction assembly; gap junction assembly; protein heterooligomerization; axon regeneration; male gonad development; establishment and/or maintenance of cell polarity; actin filament organization; odontogenesis of dentine-containing teeth; muscle cell differentiation; ovarian follicle development; negative regulation of neuroblast proliferation; response to estrogen stimulus; positive regulation of smoothened signaling pathway; positive regulation of muscle cell differentiation; vascular endothelial growth factor receptor signaling pathway; cell adhesion; aging