CRYZ sirna

CRYZ siRNA (Human)

Cat. Num. AAA8228250

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRYZ; CRYZ siRNA (Human); Quinone oxidoreductase; NADPH:quinone reductase; Zeta-crystallin; CRYZ sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CRYZ siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 329

• Applicable Applications for CRYZ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CRYZ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRYZ sirna

siRNA to inhibit CRYZ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 194239674
• NCBI GeneID: 1429
• NCBI Accession #: NP_001123514.1
• NCBI GenBank Nucleotide #: NM_001130042.1
• UniProt Accession #: Q08257; Q53FT0; Q59EU7; Q5HYE7; Q6NSK9; A6NN60; D3DQ76
• Molecular Weight: 31,528 Da
• NCBI Official Full Name: quinone oxidoreductase isoform a
• NCBI Official Synonym Full Names: crystallin, zeta (quinone reductase)
• NCBI Official Symbol: CRYZ
• NCBI Protein Information: quinone oxidoreductase
• UniProt Protein Name: Quinone oxidoreductase
• Protein Family: Zeta-crystallin
• UniProt Gene Name: CRYZ
• UniProt Entry Name: QOR_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. Unlike other mammalian species, in humans, lens expression is low. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One pseudogene is known to exist. [provided by RefSeq, Sep 2008]

Uniprot Description

CRYZ: Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; RNA-binding; EC 1.6.5.5

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; cytosol

Molecular Function: mRNA 3'-UTR binding; zinc ion binding; NADPH:quinone reductase activity

Biological Process: visual perception; protein homotetramerization; xenobiotic catabolic process