CRX sirna

CRX siRNA (Human)

Cat. Num. AAA8226942

• Gene Names: CRX; CRD; LCA7; OTX3; CORD2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRX; CRX siRNA (Human); CORD2; Cone-rod homeobox protein; CRX sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CRX siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 299

• Applicable Applications for CRX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CRX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRX sirna

siRNA to inhibit CRX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4557489
• NCBI GeneID: 1406
• NCBI Accession #: NP_000545.1
• NCBI GenBank Nucleotide #: NM_000554.4
• UniProt Accession #: O43186; Q0QD45
• Molecular Weight: 32,261 Da
• NCBI Official Full Name: cone-rod homeobox protein
• NCBI Official Synonym Full Names: cone-rod homeobox
• NCBI Official Symbol: CRX
• NCBI Official Synonym Symbols: CRD; LCA7; OTX3; CORD2
• NCBI Protein Information: cone-rod homeobox protein
• UniProt Protein Name: Cone-rod homeobox protein
• Protein Family: Cone-rod homeobox protein
• UniProt Gene Name: CRX
• UniProt Synonym Gene Names: CORD2
• UniProt Entry Name: CRX_HUMAN

NCBI Description

The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CRX: Binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, e.g. NRL and RX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors. Defects in CRX are the cause of Leber congenital amaurosis type 7 (LCA7). LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children. Defects in CRX are the cause of cone-rod dystrophy type 2 (CORD2); also known as cone-rod retinal dystrophy 2 (CRD2). CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa. Defects in CRX are a cause of retinitis pigmentosa (RP). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Belongs to the paired homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein binding; leucine zipper domain binding; chromatin binding; transcription factor activity; nuclear hormone receptor binding

Biological Process: circadian rhythm; organ morphogenesis; visual perception; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of photoreceptor cell differentiation; retina development in camera-type eye; response to stimulus; positive regulation of transcription from RNA polymerase II promoter

Disease: Cone-rod Dystrophy 2; Leber Congenital Amaurosis 7; Retinitis Pigmentosa