COX18 sirna

COX18 siRNA (Human)

Cat. Num. AAA8222387

• Gene Names: COX18; COX18HS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COX18; COX18 siRNA (Human); OXA1L2; Mitochondrial inner membrane protein COX18; COX18Hs; Cytochrome c oxidase assembly protein 18; COX18 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: COX18 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 182

• Applicable Applications for COX18 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COX18 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COX18 sirna

siRNA to inhibit COX18 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 663071143
• NCBI GeneID: 285521
• NCBI Accession #: NP_001284662.1
• NCBI GenBank Nucleotide #: NM_001297733.1
• UniProt Accession #: Q8N8Q8; Q2PB66; Q2PB67; Q2PB68; Q49B97; Q6IPP9
• Molecular Weight: 15,822 Da
• NCBI Official Full Name: mitochondrial inner membrane protein COX18 isoform 3
• NCBI Official Synonym Full Names: COX18 cytochrome c oxidase assembly factor
• NCBI Official Symbol: COX18
• NCBI Official Synonym Symbols: COX18HS
• NCBI Protein Information: mitochondrial inner membrane protein COX18
• UniProt Protein Name: Mitochondrial inner membrane protein COX18
• Protein Family: Mitochondrial inner membrane protein
• UniProt Gene Name: COX18
• UniProt Synonym Gene Names: OXA1L2; COX18Hs
• UniProt Entry Name: COX18_HUMAN

NCBI Description

This gene encodes a cytochrome c oxidase assembly protein. The encoded protein is essential for integral membrane protein insertion into the mitochondrial inner membrane. It is also required for cytochrome c oxidase assembly and activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

COX18: Required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome c oxidase. Plays a central role in the translocation and export of the C-terminal part of the COX2 protein into the mitochondrial intermembrane space. Belongs to the OXA1/ALB3/YidC family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: integral to mitochondrial inner membrane

Molecular Function: protein transporter activity

Biological Process: protein transport; protein insertion into mitochondrial membrane; respiratory chain complex IV assembly