COX11 sirna

COX11 siRNA (Human)

Cat. Num. AAA8226535

• Gene Names: COX11; COX11P
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COX11; COX11 siRNA (Human); Cytochrome c oxidase assembly protein COX11 mitochondrial; COX11 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: COX11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 231

• Applicable Applications for COX11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human COX11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COX11 sirna

siRNA to inhibit COX11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4758034
• NCBI GeneID: 1353
• NCBI Accession #: NP_004366.1
• NCBI GenBank Nucleotide #: NM_004375.3
• UniProt Accession #: Q9Y6N1; Q6FHB7; Q9BRX0; Q9UME8; D3DTY5; I3L220
• Molecular Weight: 26,133 Da
• NCBI Official Full Name: cytochrome c oxidase assembly protein COX11, mitochondrial isoform 1
• NCBI Official Synonym Full Names: COX11 cytochrome c oxidase copper chaperone
• NCBI Official Symbol: COX11
• NCBI Official Synonym Symbols: COX11P
• NCBI Protein Information: cytochrome c oxidase assembly protein COX11, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase assembly protein COX11, mitochondrial
• Protein Family: Cytochrome c oxidase assembly protein
• UniProt Gene Name: COX11
• UniProt Entry Name: COX11_HUMAN

NCBI Description

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6. [provided by RefSeq, Jun 2009]

Uniprot Description

COX11: Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I. Belongs to the COX11/CtaG family.

Protein type: Oxidoreductase; Membrane protein, integral; Mitochondrial; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: cytochrome-c oxidase activity; copper ion binding; electron carrier activity

Biological Process: negative regulation of glucokinase activity; respiratory chain complex IV assembly; respiratory gaseous exchange