CNST sirna

CNST siRNA (Human)

Cat. Num. AAA8220487

• Gene Names: CNST; C1orf71; PPP1R64
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CNST; CNST siRNA (Human); C1orf71; Consortin; CNST sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CNST siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 616

• Applicable Applications for CNST sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CNST gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CNST sirna

siRNA to inhibit CNST expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 213021162
• NCBI GeneID: 163882
• NCBI Accession #: NP_001132931.1
• NCBI GenBank Nucleotide #: NM_001139459.1
• UniProt Accession #: Q6PJW8; Q5VSY9; Q5VTM7; Q8IYA9; Q8N3L5; Q8NB09; Q8TEI2; Q96MR5
• Molecular Weight: 29,306 Da
• NCBI Official Full Name: consortin isoform 2
• NCBI Official Synonym Full Names: consortin, connexin sorting protein
• NCBI Official Symbol: CNST
• NCBI Official Synonym Symbols: C1orf71; PPP1R64
• NCBI Protein Information: consortin
• UniProt Protein Name: Consortin
• Protein Family: Consortin
• UniProt Gene Name: CNST
• UniProt Synonym Gene Names: C1orf71
• UniProt Entry Name: CNST_HUMAN

NCBI Description

Targeting of numerous transmembrane proteins to the cell surface is thought to depend on their recognition by cargo receptors that interact with the adaptor machinery for anterograde traffic at the distal end of the Golgi complex. Consortin (CNST) is an integral membrane protein that acts as a binding partner of connexins, the building blocks of gap junctions, and acts as a trans-Golgi network (TGN) receptor involved in connexin targeting to the plasma membrane and recycling from the cell surface (del Castillo et al., 2010 [PubMed 19864490]).[supplied by OMIM, Jun 2010]

Uniprot Description

CNST: Required for targeting of connexins to the plasma membrane. Belongs to the CNST family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: transport vesicle; protein complex; membrane; integral to membrane; plasma membrane; trans-Golgi network

Molecular Function: protein binding; phosphatase binding

Biological Process: positive regulation of Golgi to plasma membrane protein transport