CNEP1R1 sirna

CNEP1R1 siRNA (Human)

Cat. Num. AAA8234847

• Gene Names: CNEP1R1; TMP125; NEP1-R1; TMEM188; C16orf69
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CNEP1R1; CNEP1R1 siRNA (Human); C16orf69; TMEM188; Nuclear envelope phosphatase-regulatory subunit 1; NEP1-R1; Transmembrane protein 188; CNEP1R1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CNEP1R1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 125

• Applicable Applications for CNEP1R1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CNEP1R1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CNEP1R1 sirna

siRNA to inhibit CNEP1R1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 529250119
• NCBI GeneID: 255919
• NCBI Accession #: NP_001268718.1
• NCBI GenBank Nucleotide #: NM_001281789.1
• UniProt Accession #: Q8N9A8; Q4G1A9; Q5H9V0; Q8NE06
• Molecular Weight: 5,618 Da
• NCBI Official Full Name: nuclear envelope phosphatase-regulatory subunit 1 isoform 2
• NCBI Official Synonym Full Names: CTD nuclear envelope phosphatase 1 regulatory subunit 1
• NCBI Official Symbol: CNEP1R1
• NCBI Official Synonym Symbols: TMP125; NEP1-R1; TMEM188; C16orf69
• NCBI Protein Information: nuclear envelope phosphatase-regulatory subunit 1
• UniProt Protein Name: Nuclear envelope phosphatase-regulatory subunit 1
• Protein Family: Nuclear envelope phosphatase-regulatory
• UniProt Gene Name: CNEP1R1
• UniProt Synonym Gene Names: C16orf69; TMEM188; NEP1-R1
• UniProt Entry Name: NEPR1_HUMAN

NCBI Description

This gene encodes a transmembrane protein that belongs to the Tmemb_18A family. A similar protein in yeast is a component of an endoplasmic reticulum-associated protein phosphatase complex and is thought to play a role in the synthesis of triacylglycerol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

TMEM188: Forms with the serine/threonine protein phosphatase CTDNEP1 an active complex which dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at differents levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. Belongs to the CNEP1R1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: nuclear membrane; cytoplasm; integral to membrane; nuclear envelope

Molecular Function: protein binding

Biological Process: mitotic nuclear envelope disassembly; mitotic cell cycle; lipid metabolic process; positive regulation of protein amino acid dephosphorylation