CMAS sirna

CMAS siRNA (Human)

Cat. Num. AAA8218298

• Gene Names: CMAS; CSS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CMAS; CMAS siRNA (Human); N-acylneuraminate cytidylyltransferase; CMP-N-acetylneuraminic acid synthase; CMP-NeuNAc synthase; CMAS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CMAS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 434

• Applicable Applications for CMAS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CMAS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CMAS sirna

siRNA to inhibit CMAS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8923900
• NCBI GeneID: 55907
• NCBI Accession #: NP_061156.1
• NCBI GenBank Nucleotide #: NM_018686.4
• UniProt Accession #: Q8NFW8; Q96AX5; Q9NQZ0
• Molecular Weight: 29,561 Da
• NCBI Official Full Name: N-acylneuraminate cytidylyltransferase
• NCBI Official Synonym Full Names: cytidine monophosphate N-acetylneuraminic acid synthetase
• NCBI Official Symbol: CMAS
• NCBI Official Synonym Symbols: CSS
• NCBI Protein Information: N-acylneuraminate cytidylyltransferase
• UniProt Protein Name: N-acylneuraminate cytidylyltransferase
• Protein Family: N-acylneuraminate cytidylyltransferase
• UniProt Gene Name: CMAS
• UniProt Synonym Gene Names: CMP-NeuNAc synthase
• UniProt Entry Name: NEUA_HUMAN

NCBI Description

Sialic acids are a family of nine-carbon sugars on cell surface glycoproteins and glycolipids that play a pivotal role in determining the structure and function of many animal tissues. The pattern of cell surface sialylation is highly regulated during embryonic development and N-glycosylation is a common post-translational modification during cellular differentiation. Sialic acids play important roles in cell-cell communications and immune responses. Sialylated glycoprotein and glycolipid formation requires the activation of a sialic acid to a cytidine monophosphate (CMP) diester by the enzyme encoded by this gene: CMP-N-acetylneuraminic acid synthetase. [provided by RefSeq, Jul 2012]

Uniprot Description

CMAS: Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Belongs to the CMP-NeuNAc synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.43; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: N-acylneuraminate cytidylyltransferase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; N-acetylneuraminate metabolic process