CLECL1 sirna

CLECL1 siRNA (Human)

Cat. Num. AAA8227273

• Gene Names: CLECL1; DCAL1; DCAL-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CLECL1; CLECL1 siRNA (Human); DCAL1; C-type lectin-like domain family 1; Dendritic cell-associated lectin 1; DC-associated lectin-1; DCAL-1; CLECL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CLECL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 167

• Applicable Applications for CLECL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLECL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CLECL1 sirna

siRNA to inhibit CLECL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 359279935
• NCBI GeneID: 160365
• NCBI Accession #: NP_001240679.1
• NCBI GenBank Nucleotide #: NM_001253750.1
• UniProt Accession #: Q8IZS7
• Molecular Weight: 19,115 Da
• NCBI Official Full Name: C-type lectin-like domain family 1 isoform 3
• NCBI Official Synonym Full Names: C-type lectin-like 1
• NCBI Official Symbol: CLECL1
• NCBI Official Synonym Symbols: DCAL1; DCAL-1
• NCBI Protein Information: C-type lectin-like domain family 1
• UniProt Protein Name: C-type lectin-like domain family 1
• Protein Family: C-type lectin-like domain family
• UniProt Gene Name: CLECL1
• UniProt Synonym Gene Names: DCAL1; DC-associated lectin-1; DCAL-1
• UniProt Entry Name: CLCL1_HUMAN

NCBI Description

This gene encodes a type II transmembrane, C-type lectin-like protein that is highly expressed on dendritic and B cells. This protein may act as a T-cell costimulatory molecule that enhances interleukin-4 production, and maybe involved in the regulation of the immune response. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CLECL1: May function in mediating immune cell-cell interactions. May act as a T-cell costimulatory molecule, enhancing anti-CD3- induced proliferation. May play a role in the interaction of dendritic cells with T-cells and the cells of the adaptive immune response.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: plasma membrane; integral to membrane

Molecular Function: carbohydrate binding